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Literature summary for 3.1.4.39 extracted from

  • Sugimoto, S.; Sugimoto, H.; Aoyama, C.; Aso, C.; Mori, M.; Izumi, T.
    Purification and characterization of lysophospholipase D from rat brain (2006), Biochim. Biophys. Acta, 1761, 1410-1418.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA hydrolysis of 1-palmitoyl-glycerophoshorylcholine is inhibited, indicating that a divalent cation is essential for catalytic activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information suggestion that lysoPLD predominantly utilizes saturated forms of lysophosphatidylcholine Rattus norvegicus
0.0083
-
1-linoleoyl-2-lyso-glycerophoshorylcholine Vmax is 0.019 micromol/min/mg Rattus norvegicus
0.0207
-
1-palmitoyl-2-lyso-glycerophoshorylcholine Vmax is 0.059 micromol/min/mg Rattus norvegicus
0.0267
-
1-O-hexadecyl-2-lyso-glycerophosphorylcholine Vmax is 0.29 micromol/min/mg, lysoPLD activity is more than twice that observed with 1-palmitoyl-glycerophoshorylcholine Rattus norvegicus
0.101
-
1-stearoyl-2-lyso-glycerophoshorylcholine Vmax is 0.035 micromol/min/mg Rattus norvegicus
0.176
-
1-palmitoyl-glycerophoshorylcholine Vmax is 0.3 micromol/min/mg, best substrate Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Rattus norvegicus 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ purified enzyme is incubated with Co2+. LysoPLD activity in serum is more sensitive to Co2+ Rattus norvegicus
Mg2+ the purified enzyme is incubated with Mg2+. Purified enzyme is more sensitive to Mg2+ than to Co2+. Enzyme activity requires Mg2+ Rattus norvegicus
Mn2+ the purified enzyme is incubated with Mn2+ Rattus norvegicus
Na3VO4 enzyme activity requires Na3VO4, not necessary after purification Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
lysoPLD, not identical to 100 kDa autotaxin protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lysophosphatidylcholine + H2O Rattus norvegicus
-
lysophosphatidic acid + choline
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
includes a protease inhibitor at all steps to avoid isolation of the products of proteolysis. Purified to apparent homogeneity from rat brain nuclear fractions using 1-[14C]palmitoyl-glycerophosphorylcholine as a substrate Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
nuclear fraction has relatively high levels of lysoPLD activity. Both lysoPLD activity and highest band density found in microsomal fraction Rattus norvegicus
0.036
-
using isotopic assay Rattus norvegicus
0.048
-
colorimetric assay, 6 h incubation, 37°C Rattus norvegicus
0.14
-
purified enzyme, HiTrap DEAE FF Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-linoleoyl-2-lyso-glycerophoshorylcholine + H2O
-
Rattus norvegicus 1-linoleoyl-sn-glycerol-3-phosphate + choline
-
?
1-O-hexadecyl-2-lyso-glycerophosphorylcholine + H2O lysoPAF (alkyl-lysophosphatidylcholine), lysoPLD shows higher activity towards lysoPAF Rattus norvegicus 1-O-hexadecyl-sn-glycerol-3-phosphate + choline
-
?
1-palmitoyl-2-lyso-glycerophoshorylcholine + H2O
-
Rattus norvegicus 1-palmitoyl-sn-glycerol-3-phosphate + choline
-
?
1-stearoyl-2-lyso-glycerophoshorylcholine + H2O
-
Rattus norvegicus 1-stearoyl-sn-glycerol-3-phosphate + choline
-
?
lysophosphatidylcholine + H2O
-
Rattus norvegicus lysophosphatidic acid + choline
-
?
lysophosphatidylcholine + H2O LPC, purified enzyme hydrolyzes saturated forms of lysophosphatidylcholine more robustly than unsaturated forms Rattus norvegicus lysophosphatic acid + choline
-
?
platelet-activating factor + H2O PAF, same extent as palmityl-lysophosphatidylcholine Rattus norvegicus ? + choline
-
?

Subunits

Subunits Comment Organism
monomer
-
Rattus norvegicus

Synonyms

Synonyms Comment Organism
lyso-PLD
-
Rattus norvegicus
lysophospholipase D
-
Rattus norvegicus
lysoPLD purified from rat brain nucleus fractions, has the ability to hydrolyze the ester-bond between phoshate and choline in 1-palmitoyl-glycerophoshorylcholine Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 highest enzyme activity Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Rattus norvegicus