Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | hydrolysis of 1-palmitoyl-glycerophoshorylcholine is inhibited, indicating that a divalent cation is essential for catalytic activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | suggestion that lysoPLD predominantly utilizes saturated forms of lysophosphatidylcholine | Rattus norvegicus | |
0.0083 | - |
1-linoleoyl-2-lyso-glycerophoshorylcholine | Vmax is 0.019 micromol/min/mg | Rattus norvegicus | |
0.0207 | - |
1-palmitoyl-2-lyso-glycerophoshorylcholine | Vmax is 0.059 micromol/min/mg | Rattus norvegicus | |
0.0267 | - |
1-O-hexadecyl-2-lyso-glycerophosphorylcholine | Vmax is 0.29 micromol/min/mg, lysoPLD activity is more than twice that observed with 1-palmitoyl-glycerophoshorylcholine | Rattus norvegicus | |
0.101 | - |
1-stearoyl-2-lyso-glycerophoshorylcholine | Vmax is 0.035 micromol/min/mg | Rattus norvegicus | |
0.176 | - |
1-palmitoyl-glycerophoshorylcholine | Vmax is 0.3 micromol/min/mg, best substrate | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | purified enzyme is incubated with Co2+. LysoPLD activity in serum is more sensitive to Co2+ | Rattus norvegicus | |
Mg2+ | the purified enzyme is incubated with Mg2+. Purified enzyme is more sensitive to Mg2+ than to Co2+. Enzyme activity requires Mg2+ | Rattus norvegicus | |
Mn2+ | the purified enzyme is incubated with Mn2+ | Rattus norvegicus | |
Na3VO4 | enzyme activity requires Na3VO4, not necessary after purification | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
lysoPLD, not identical to 100 kDa autotaxin protein | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | Rattus norvegicus | - |
lysophosphatidic acid + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
includes a protease inhibitor at all steps to avoid isolation of the products of proteolysis. Purified to apparent homogeneity from rat brain nuclear fractions using 1-[14C]palmitoyl-glycerophosphorylcholine as a substrate | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
nuclear fraction has relatively high levels of lysoPLD activity. Both lysoPLD activity and highest band density found in microsomal fraction | Rattus norvegicus |
0.036 | - |
using isotopic assay | Rattus norvegicus |
0.048 | - |
colorimetric assay, 6 h incubation, 37°C | Rattus norvegicus |
0.14 | - |
purified enzyme, HiTrap DEAE FF | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-linoleoyl-2-lyso-glycerophoshorylcholine + H2O | - |
Rattus norvegicus | 1-linoleoyl-sn-glycerol-3-phosphate + choline | - |
? | |
1-O-hexadecyl-2-lyso-glycerophosphorylcholine + H2O | lysoPAF (alkyl-lysophosphatidylcholine), lysoPLD shows higher activity towards lysoPAF | Rattus norvegicus | 1-O-hexadecyl-sn-glycerol-3-phosphate + choline | - |
? | |
1-palmitoyl-2-lyso-glycerophoshorylcholine + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycerol-3-phosphate + choline | - |
? | |
1-stearoyl-2-lyso-glycerophoshorylcholine + H2O | - |
Rattus norvegicus | 1-stearoyl-sn-glycerol-3-phosphate + choline | - |
? | |
lysophosphatidylcholine + H2O | - |
Rattus norvegicus | lysophosphatidic acid + choline | - |
? | |
lysophosphatidylcholine + H2O | LPC, purified enzyme hydrolyzes saturated forms of lysophosphatidylcholine more robustly than unsaturated forms | Rattus norvegicus | lysophosphatic acid + choline | - |
? | |
platelet-activating factor + H2O | PAF, same extent as palmityl-lysophosphatidylcholine | Rattus norvegicus | ? + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
lyso-PLD | - |
Rattus norvegicus |
lysophospholipase D | - |
Rattus norvegicus |
lysoPLD | purified from rat brain nucleus fractions, has the ability to hydrolyze the ester-bond between phoshate and choline in 1-palmitoyl-glycerophoshorylcholine | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | highest enzyme activity | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Rattus norvegicus |