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Literature summary for 3.1.4.37 extracted from

  • Hinman, J.; Chen, C.; Oh, S.; Hollander, W.; Abraham, C.
    Age-dependent accumulation of ubiquitinated 2',3'-cyclic nucleotide 3'-phosphodiesterase in myelin lipid rafts (2008), Glia, 56, 118-133.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
GC-CNP encoding 155–238 aa of GFP fused to the N-terminus of CNP co-transfected with YN-Ub into COS-7 cells Chlorocebus aethiops
GC-CNP encoding 155–238 aa of GFP fused to the N-terminus of CNP co-transfected with YN-Ub into MO3.13 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information partially localize within lipid rafts Homo sapiens
-
-
additional information partially localize within lipid rafts Chlorocebus aethiops
-
-
additional information ubiquitinated CNP in the Triton X-100 insoluble lipid raft associated fractions of myelin Macaca mulatta
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
in lipid raft fraction from an aged monkey, evidence of sequential CNP proteolysis, abundance of CNP immunoreactive higher molecular weight material Macaca mulatta
40000
-
2D electrophoresis and immunoblotting, lipid raft fraction from a young monkey Macaca mulatta
46000
-
endogenous CNP, immunoblot analysis Homo sapiens
48000
-
endogenous CNP, immunoblot analysis Homo sapiens
50000
-
2D electrophoresis and immunoblotting, lipid raft fraction from a young monkey Macaca mulatta
50000
-
GC-CNP transfected MO3.13 cells, immunoblot analysis Homo sapiens
60000
-
GC-CNP transfected MO3.13 cells, immunoblot analysis Homo sapiens
150000
-
GC-CNP/YN-Ub complex transfected MO3.13 cells, immunoblot analysis Homo sapiens
250000
-
GC-CNP/YN-Ub complex transfected MO3.13 cells, immunoblot analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Chlorocebus aethiops
-
-
-
Homo sapiens
-
-
-
Macaca mulatta
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Macaca mulatta
-
cerebral white matter with age, CNP accumulates throughout brain white matter accompanied by proteolytic fragments of CNP. Equal increase in both isoforms CNP1 and CNP2 Macaca mulatta
-
COS-7 cell ubiquitinated CNP Chlorocebus aethiops
-
additional information MO3.13 cell, ubiquitinated CNP Homo sapiens
-
myelin with age, CNP accumulates in myelin accompanied by proteolytic fragments of CNP. Equal increase in both isoforms CNP1 and CNP2 Macaca mulatta
-
oligodendrocyte
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
2',3'-cyclic nucleotide 3'-phosphodiesterase
-
Homo sapiens
2',3'-cyclic nucleotide 3'-phosphodiesterase
-
Macaca mulatta
2',3'-cyclic nucleotide 3'-phosphodiesterase
-
Chlorocebus aethiops
CNP
-
Homo sapiens
CNP
-
Macaca mulatta
CNP
-
Chlorocebus aethiops

pI Value

Organism Comment pI Value Maximum pI Value
Macaca mulatta 2D electrophoresis and immunoblotting, lipid raft fraction from a young monkey
-
9

Expression

Organism Comment Expression
Macaca mulatta CNP mRNA levels do not change with age additional information

General Information

General Information Comment Organism
physiological function an age-related impairment in proteasomal proteolysis and subsequent accumulation of ubiquitinated CNP activates alternative proteolytic mechanisms leading to CNP fragmentation Chlorocebus aethiops
physiological function an age-related impairment in proteasomal proteolysis and subsequent accumulation of ubiquitinated CNP activates alternative proteolytic mechanisms leading to CNP fragmentation. In the aged monkey, CNP is ubiquitinated and this ubiquitination can be found (at least in part) in detergent insoluble membrane microdomains Homo sapiens
physiological function incomplete degradation of CNP due to failure of the proteasomal system and aberrant degradation by calpain-1 leads to agerelated CNP accumulation and proteolysis. These phenomena result in age-related dysfunction of CNP in the lipid raft, which may lead to myelin and axonal pathology Macaca mulatta