Cloned (Comment) | Organism |
---|---|
subcloned into pTH27, expressed in Escherichia coli Rosetta (DE3) with a TEV protease-cleavable His-tag | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.537 | - |
2',3'-cAMP | full-length protein | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | assay buffer | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26700 | - |
x * 26700, SDS-PAGE, C-terminal domain. x * 45200, SDS-PAGE, full-length CNPase | Mus musculus |
45200 | - |
x * 26700, SDS-PAGE, C-terminal domain. x * 45200, SDS-PAGE, full-length CNPase | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-affinity chromatography followed by gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myelin sheath | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
4°C, for weeks | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | the protein consists of two domains: an uncharacterized N-terminal domain with little homology to other proteins, and a C-terminal phosphodiesterase domain. C-terminal tail (22 residues) has no significant effect on the catalytic activity, neither do the first 24 N-terminal residues of the full-length protein. Both the N- and C-terminal domain of recombinant CNPase are folded entities: N-terminal domain has more beta-sheet structure and less alpha-helices than the C-terminal domain | Mus musculus | 2'-AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26700, SDS-PAGE, C-terminal domain. x * 45200, SDS-PAGE, full-length CNPase | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
2',3'-cyclic nucleotide 3'-phosphodiesterase | - |
Mus musculus |
CNPase | - |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 75 | increase in the melting temperature induced by both pH 5.5 and high salt concentration. At pH 5.5, without NaCl: ca. 68°C and with 500 mM NaCl: ca. 75°C. At pH 7.5 without NaCl and with 500 mM NaCl: ca. 70°C | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
118 | - |
2',3'-cAMP | full-length protein | Mus musculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
in pH 5.5 and in the presence of 500 mM NaCl, CNPase is much more stable than at neutral pH with lower salt content | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
220 | - |
2',3'-cAMP | full-length protein | Mus musculus |