Literature summary for 3.1.4.35 extracted from

Biswas, K.H.; Sopory, S.; Visweswariah, S.S.
The GAF domain of the cGMP-binding, cGMP-specific phosphodiesterase (PDE5) is a sensor and a sink for cGMP (2008), Biochemistry, 47, 3534-3543.

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Application

Application	Comment	Organism
analysis	fusion protein of wild-type and mutant F163A GAFa domain of enzyme to Green Fluorescent Protein or Renilla luciferase for use in bioluminescence resonance energy transfer assay BRET as a biosensor of cGMP. BRET ratios of wild-type, but not mutant F163A, increase in presence of cGMP, but not cAMP	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
fusion protein of wild-type and mutant F163A GAFa domain of enzyme to Green Fluorescent Protein or Renilla luciferase	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F163A	mutantion in GAFa domain of enzyme, mutant is unable to bind cGMP	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O76074	isoform PDE5A	-

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Release 2023.1 (January 2023)