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Literature summary for 3.1.4.35 extracted from

  • Huang, D.; Hinds, T.R.; Martinez, S.E.; Doneanu, C.; Beavo, J.A.
    Molecular determinants of cGMP binding to chicken cone photoreceptor phosphodiesterase (2004), J. Biol. Chem., 279, 48143-48151.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gallus gallus

Protein Variants

Protein Variants Comment Organism
D166A mutation reduces cGMP affinity Gallus gallus
D169A mutant enzyme shows no binding of cGMP Gallus gallus
E124A mutation reduces cGMP affinity Gallus gallus
F123A mutant enzyme shows no binding of cGMP Gallus gallus
F123W mutant enzyme shows no binding of cGMP Gallus gallus
F123Y mutation reduces cGMP affinity Gallus gallus
K170A mutation reduces cGMP affinity Gallus gallus
S165A mutation reduces cGMP affinity Gallus gallus
S97A mutation reduces cGMP affinity Gallus gallus
T172A mutant enzyme shows no binding of cGMP Gallus gallus
T175A mutation reduces cGMP affinity Gallus gallus
T176A mutant enzyme shows no binding of cGMP Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.717
-
3',5'-cAMP enzyme from cone Gallus gallus
0.82
-
3',5'-cAMP enzyme from rod Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus P52731
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O very low activity Gallus gallus 5'-AMP
-
?
3',5'-cGMP + H2O
-
Gallus gallus 5'-GMP
-
?

Synonyms

Synonyms Comment Organism
PDE6
-
Gallus gallus