Literature summary for 3.1.4.35 extracted from

Sung, B.J.; Hwang, K.Y.; Jeon, Y.H.; Lee, J.I.; Heo, Y.S.; Kim, J.H.; Moon, J.; Yoon, J.M.; Hyun, Y.L.; Kim, E.; Eum, S.J.; Park, S.Y.; Lee, J.O.; Lee, T.G.; Ro, S.; Cho, J.M.
Structure of the catalytic domain of human phosphodiesterase 5 with bound drug molecules (2003), Nature, 425, 98-102.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the catalytic domain of PDE5A1 in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of PDE5A1 complexed with the inhibitors sildenafil, tadalafil or vardenafil, PDE5A1-inhibitor complex crystals are grown at 4°C by hanging-drop vapour diffusion, adding 0.001 ml protein solution containing 10 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 100 mM NaCl and 5 mM dithiothreitol, to 0.001 ml well solution consisting of 100 mM Tris-HCl, pH 7.2-7.5, 200 mM MgCl2 and 6-9% polyethylene glycol 8000, crystals diffract to 2.3-2.8 A	Homo sapiens

Crystallization (Comment)

Organism

crystals of PDE5A1 complexed with the inhibitors sildenafil, tadalafil or vardenafil, PDE5A1-inhibitor complex crystals are grown at 4°C by hanging-drop vapour diffusion, adding 0.001 ml protein solution containing 10 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 100 mM NaCl and 5 mM dithiothreitol, to 0.001 ml well solution consisting of 100 mM Tris-HCl, pH 7.2-7.5, 200 mM MgCl2 and 6-9% polyethylene glycol 8000, crystals diffract to 2.3-2.8 A

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
1-[[3-(6,7-dihydro-1-methyl-7-oxo-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-5-yl)-4-ethoxyphenyl]sulfonyl]-4-methylpiperazine citrate	trivial name sildenafil citrate or Viagra	Homo sapiens
2-[2-ethoxy-5-(4-ethyl-piperazine-1-sulfonyl)phenyl]-5-methyl-7-propyl-3H-imidazo[5,1-f][1,2,4]triazin-4-one	trivial name vardenafil or Levitra	Homo sapiens
6-benzo[1,3]dioxol-5-yl-2-methyl-2,3,6,7,12,12a-hexahydropyrazinol[1',2':1,6]pyrido[3,4-b]indole-1,4-dione	trivial name tadalafil or Cialis	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
guanosine 3',5'-cyclic phosphate + H2O	Homo sapiens	-	guanosine 5'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O76074	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic domain of PDE5A1	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
corpus cavernosum	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
guanosine 3',5'-cyclic phosphate + H2O	-	Homo sapiens	guanosine 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
cGMP-specific PDE5	-	Homo sapiens
PDE5A1	-	Homo sapiens
phosphodiesterase 5	-	Homo sapiens