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Literature summary for 3.1.4.35 extracted from

  • Giordano, D.; De Stefano, M.E.; Citro, G.; Modica, A.; Giorgi, M.
    Expression of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in mouse tissues and cell lines using an antibody against the enzyme amino-terminal domain (2001), Biochim. Biophys. Acta, 1539, 16-27.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
sildenafil 0.0000035 mM, 50% inhibition Mus musculus
zaprinast 0.0006 mM, 50% inhibition Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
x * 98000, immunoblot Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine 3',5'-cyclic phosphate + H2O Mus musculus
-
guanosine 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q, gel filtration, partially purified from N18TG2 and NG108-15 neuroblastoma cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain low expression Mus musculus
-
cerebellum
-
Mus musculus
-
heart
-
Mus musculus
-
kidney low expression Mus musculus
-
liver very low expression Mus musculus
-
lung highest PDE5 expression Mus musculus
-
neuroblastoma cell PDE5 is induced in neuroblastoma-glioma NG108-15 cells after dibutyryl treatment Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00061
-
partially purified PDE5 from N18TG2 neuroblastoma cells Mus musculus
1.44
-
partially purified PDE5 from NG108-15 neuroblastoma cells Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 3',5'-cyclic phosphate + H2O
-
Mus musculus guanosine 5'-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 98000, immunoblot Mus musculus

Synonyms

Synonyms Comment Organism
cGMP-binding cGMP-specific phosphodiesterase
-
Mus musculus
PDE5
-
Mus musculus