Literature summary for 3.1.4.35 extracted from

Okada, D.; Asakawa, S.
Allosteric activation of cGMP-specific, cGMP-binding phosphodiesterase (PDE5) by cGMP (2002), Biochemistry, 41, 9672-9679.

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Activating Compound

Activating Compound	Comment	Organism	Structure
cGMP	allosteric activation	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	cGMP	37°C, pH 7.4	Rattus norvegicus
0.012	-	2'-O-anthraniloyl cGMP	37°C, pH 7.4	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
guanosine 3',5'-cyclic phosphate + H2O	Rattus norvegicus	-	guanosine 5'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose, Blue-Sepharose, calmodulin-conjugated Sepharose, partially purified	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cerebellum	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000012	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2'-O-anthraniloyl cGMP + H2O	-	Rattus norvegicus	2'-O-anthraniloyl GMP	-	?
guanosine 3',5'-cyclic phosphate + H2O	-	Rattus norvegicus	guanosine 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
cGMP-specific cGMP-binding phosphodiesterase	-	Rattus norvegicus
PDE5	-	Rattus norvegicus

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Release 2023.1 (January 2023)