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Literature summary for 3.1.4.35 extracted from

  • Francis, S.H.; Turko, I.V.; Grimes, K.A.; Corbin, J.D.
    Histidine-607 and histidine-643 provide important interactions for metal support of catalysis in phosphodiesterase-5 (2000), Biochemistry, 39, 9591-9596.
    View publication on PubMed
Search for more literature for 3.1.4.35

Cloned(Commentary)

Cloned (Comment) Organism
expression of PDE5 in Sf9 insect cells Bos taurus

Protein Variants

Protein Variants Comment Organism
D644A 10% and 4% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
D714A 33% and 22% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
D754A 59% and 28% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
E632A 20% and 16% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
E672A 3% and 8% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
H603A 38% and 22% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
H607A 3.9% and 13% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
H643A 3% and less than 1% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
H647A less than 1% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
H675A 9% and 4% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
N604A less than 2% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ approx. 3fold increase in activity at 10 mM Bos taurus
Mn2+ approx. 5fold increase in activity at 10 mM Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine 3',5'-cyclic phosphate + H2O Bos taurus
-
 guanosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PDE5 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 3',5'-cyclic phosphate + H2O
-
 Bos taurus guanosine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
cGMP-binding cGMP-specific PDE
-
 Bos taurus
PDE5
-
 Bos taurus