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Literature summary for 3.1.4.35 extracted from
- Cylic nucleotide phosphodiesterase in silkworm. Characterization of cyclic GMP phosphodiesterase (1975), Biochim. Biophys. Acta, 410, 310-317.
General Stability
| General Stability | Organism |
|---|---|
| 5 mM beta-mercaptoethanol and bovine serum albumin | Bombyx mori |
| mercaptoethanol, stabilization | Bombyx mori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-cGMP | - |
Bombyx mori |  |
| 3',5'-cAMP | 20% inhibition at 0.1 mM, 50% inhibition at 1 mM | Bombyx mori | |
| 3',5'-cIMP | - |
Bombyx mori | |
| ATP | slight inhibition at 1 mM | Bombyx mori | |
| Caffeine | - |
Bombyx mori | |
| dibutyryl 3',5'-cAMP | - |
Bombyx mori | |
| EDTA | completely inhibits at 10 mM | Bombyx mori | |
| GTP | slight inhibition at 1 mM | Bombyx mori | |
| theophylline | - |
Bombyx mori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
3',5'-cGMP | - |
Bombyx mori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM activates | Bombyx mori | |
| Mn2+ | 2 mM activates | Bombyx mori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | - |
gel filtration | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
silkworm | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Bombyx mori | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bombyx mori |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C | Bombyx mori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3',5'-cGMP + H2O | - |
Bombyx mori | 5'-GMP | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
with Tris-acetate as buffer | Bombyx mori |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
3',5'-cAMP | - |
Bombyx mori | |
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Release 2023.1 (January 2023)
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