Literature summary for 3.1.4.3 extracted from

Pokotylo, I.; Pejchar, P.; Potocky, M.; Kocourkova, D.; Krckova, Z.; Ruelland, E.; Kravets, V.; Martinec, J.
The plant non-specific phospholipase C gene family. Novel competitors in lipid signalling (2013), Prog. Lipid Res., 52, 62-79.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the N-terminal domain in Escherichia coli	Clostridium perfringens
phylogenetic analysis	Nicotiana tabacum
phylogenetic analysis	Glycine max
phylogenetic analysis	Sorghum bicolor
phylogenetic analysis	Oryza sativa
phylogenetic analysis	Petunia x hybrida
phylogenetic analysis	Physcomitrium patens
phylogenetic analysis	Vitis vinifera
phylogenetic analysis	Populus trichocarpa
phylogenetic analysis	Picea sitchensis
phylogenetic analysis	Selaginella moellendorffii
phylogenetic analysis	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
up	the enzyme is induced by 24-epibrassinolide signalling, auxin, cytokinin, phosphate deficiency, abscisic acid, and salt stress. NPC4 does demonstrate a positive response to Botrytis cinerea, Golovinomyces orontii, Pseudomonas syringae and Phytophthora infestans treatment	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism
additional information	no inhibition by tricyclodecan-9-ylxanthogenate, i.e. D609	Nicotiana tabacum
tricyclodecan-9-ylxanthogenate	i.e. D609; i.e. D609; i.e. D609; i.e. D609; i.e. D609; i.e. D609	Arabidopsis thaliana
tricyclodecan-9-ylxanthogenate	i.e. D609	Bacillus cereus
tricyclodecan-9-ylxanthogenate	i.e. D609	Clostridium perfringens
tricyclodecan-9-ylxanthogenate	i.e. D609	Glycine max
tricyclodecan-9-ylxanthogenate	i.e. D609	Oryza sativa
tricyclodecan-9-ylxanthogenate	i.e. D609	Petunia x hybrida
tricyclodecan-9-ylxanthogenate	i.e. D609	Physcomitrium patens
tricyclodecan-9-ylxanthogenate	i.e. D609	Picea sitchensis
tricyclodecan-9-ylxanthogenate	i.e. D609	Populus trichocarpa
tricyclodecan-9-ylxanthogenate	i.e. D609	Pseudomonas fluorescens
tricyclodecan-9-ylxanthogenate	i.e. D609	Selaginella moellendorffii
tricyclodecan-9-ylxanthogenate	i.e. D609	Sorghum bicolor
tricyclodecan-9-ylxanthogenate	i.e. D609	Ureaplasma urealyticum
tricyclodecan-9-ylxanthogenate	i.e. D609	Vitis vinifera

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
cytosol	-	Arabidopsis thaliana	5829	-
endoplasmic reticulum membrane	-	Arabidopsis thaliana	5789	-
extracellular	the enzyme is secreted	Clostridium perfringens	-	-
membrane	bound	Ureaplasma urealyticum	16020	-
membrane	of stamens and pistils	Petunia x hybrida	16020	-
mitochondrion	-	Arabidopsis thaliana	5739	-
plasma membrane	-	Nicotiana tabacum	5886	-
plasma membrane	-	Arabidopsis thaliana	5886	-
tonoplast	-	Arabidopsis thaliana	-	-
vacuolar membrane	-	Arabidopsis thaliana	5774	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Nal	activates enzyme activity	Ureaplasma urealyticum
Zn2+	bound at the N-terminal domain	Clostridium perfringens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
phosphatidylcholine + H2O	Glycine max	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Pseudomonas fluorescens	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Bacillus cereus	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Sorghum bicolor	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Oryza sativa	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Petunia x hybrida	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Clostridium perfringens	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Ureaplasma urealyticum	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Physcomitrium patens	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Vitis vinifera	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Populus trichocarpa	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Picea sitchensis	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Selaginella moellendorffii	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	Arabidopsis thaliana	-	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylethanolamine + H2O	Arabidopsis thaliana	-	1,2-sn-diacylglycerol + phosphoethanolamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	O81020	NPC2; gene NPC2	-
Arabidopsis thaliana	Q8H965	NPC6; gene NPC6	-
Arabidopsis thaliana	Q8L7Y9	NPC1; gene NPC1	-
Arabidopsis thaliana	Q9S816	NPC5; gene NPC5	-
Arabidopsis thaliana	Q9SRQ6	NPC3; gene NPC3	-
Arabidopsis thaliana	Q9SRQ7	NPC4; gene NPC4	-
Bacillus cereus	-	-	-
Clostridium perfringens	-	-	-
Glycine max	-	six genes NPC1-6	-
Nicotiana tabacum	-	-	-
Oryza sativa	-	six genes NPC1-6	-
Petunia x hybrida	-	six genes NPC1-6	-
Physcomitrium patens	-	single NPC1-like gene	-
Picea sitchensis	-	NPC1-, NPC2- and NPC6-like gene, no NPC3-5	-
Populus trichocarpa	-	six genes NPC1-6	-
Pseudomonas fluorescens	-	-	-
Selaginella moellendorffii	-	single NPC1-like gene	-
Sorghum bicolor	-	six genes NPC1-6	-
Ureaplasma urealyticum	-	-	-
Vitis vinifera	-	six genes NPC1-6	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme	Ureaplasma urealyticum

Reaction

Reaction	Comment	Organism	Reaction ID
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Nicotiana tabacum	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Glycine max	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Sorghum bicolor	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Oryza sativa	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Petunia x hybrida	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Physcomitrium patens	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Vitis vinifera	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Populus trichocarpa	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Picea sitchensis	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Selaginella moellendorffii	a
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine	mode of action of the plant enzyme family of non-specific phospholipases C, overview	Arabidopsis thaliana	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Nicotiana tabacum	-
cotyledon	-	Arabidopsis thaliana	-
inflorescence	-	Arabidopsis thaliana	-
leaf	-	Arabidopsis thaliana	-
leaf	old	Arabidopsis thaliana	-
additional information	tissue distribution, overview	Arabidopsis thaliana	-
pistil	-	Petunia x hybrida	-
pollen	germinating	Arabidopsis thaliana	-
root	-	Arabidopsis thaliana	-
seedling	higher in seedling hypocotyls and lower in seedling roots	Arabidopsis thaliana	-
silique	-	Arabidopsis thaliana	-
stamen	-	Petunia x hybrida	-
TBY-2 cell	-	Nicotiana tabacum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
phosphatidic acid + H2O	-	Arabidopsis thaliana	1,2-sn-diacylglycerol + phosphate	-	?
phosphatidylcholine + H2O	-	Glycine max	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Pseudomonas fluorescens	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Bacillus cereus	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Sorghum bicolor	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Oryza sativa	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Petunia x hybrida	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Clostridium perfringens	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Ureaplasma urealyticum	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Physcomitrium patens	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Vitis vinifera	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Populus trichocarpa	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Picea sitchensis	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Selaginella moellendorffii	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylcholine + H2O	-	Arabidopsis thaliana	1,2-sn-diacylglycerol + phosphocholine	-	?
phosphatidylethanolamine + H2O	-	Arabidopsis thaliana	1,2-sn-diacylglycerol + phosphoethanolamine	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Nicotiana tabacum
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Glycine max
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Sorghum bicolor
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Oryza sativa
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Petunia x hybrida
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Physcomitrium patens
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Vitis vinifera
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Populus trichocarpa
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Picea sitchensis
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Selaginella moellendorffii
More	the enzyme contains a central phosphoesterase domain, a DUF756 domain, a conserved region and a conserved Asp-Arg pair, and some isozymes contain a signal peptide, domain structure, overview	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
alpha-toxin	-	Clostridium perfringens
non-specific phospholipase C	-	Nicotiana tabacum
non-specific phospholipase C	-	Glycine max
non-specific phospholipase C	-	Sorghum bicolor
non-specific phospholipase C	-	Oryza sativa
non-specific phospholipase C	-	Petunia x hybrida
non-specific phospholipase C	-	Physcomitrium patens
non-specific phospholipase C	-	Vitis vinifera
non-specific phospholipase C	-	Populus trichocarpa
non-specific phospholipase C	-	Picea sitchensis
non-specific phospholipase C	-	Selaginella moellendorffii
non-specific phospholipase C	-	Arabidopsis thaliana
NPC	-	Nicotiana tabacum
NPC	-	Glycine max
NPC	-	Sorghum bicolor
NPC	-	Oryza sativa
NPC	-	Petunia x hybrida
NPC	-	Physcomitrium patens
NPC	-	Vitis vinifera
NPC	-	Populus trichocarpa
NPC	-	Picea sitchensis
NPC	-	Selaginella moellendorffii
NPC	-	Arabidopsis thaliana
NPC1	-	Glycine max
NPC1	-	Sorghum bicolor
NPC1	-	Oryza sativa
NPC1	-	Petunia x hybrida
NPC1	-	Physcomitrium patens
NPC1	-	Vitis vinifera
NPC1	-	Populus trichocarpa
NPC1	-	Picea sitchensis
NPC1	-	Selaginella moellendorffii
NPC1	-	Arabidopsis thaliana
NPC2	-	Glycine max
NPC2	-	Sorghum bicolor
NPC2	-	Oryza sativa
NPC2	-	Petunia x hybrida
NPC2	-	Vitis vinifera
NPC2	-	Populus trichocarpa
NPC2	-	Picea sitchensis
NPC2	-	Arabidopsis thaliana
NPC3	-	Glycine max
NPC3	-	Sorghum bicolor
NPC3	-	Oryza sativa
NPC3	-	Petunia x hybrida
NPC3	-	Vitis vinifera
NPC3	-	Populus trichocarpa
NPC3	-	Arabidopsis thaliana
NPC4	-	Glycine max
NPC4	-	Sorghum bicolor
NPC4	-	Oryza sativa
NPC4	-	Petunia x hybrida
NPC4	-	Vitis vinifera
NPC4	-	Populus trichocarpa
NPC4	-	Arabidopsis thaliana
NPC5	-	Glycine max
NPC5	-	Sorghum bicolor
NPC5	-	Oryza sativa
NPC5	-	Petunia x hybrida
NPC5	-	Vitis vinifera
NPC5	-	Populus trichocarpa
NPC5	-	Arabidopsis thaliana
NPC6	-	Glycine max
NPC6	-	Sorghum bicolor
NPC6	-	Oryza sativa
NPC6	-	Petunia x hybrida
NPC6	-	Vitis vinifera
NPC6	-	Populus trichocarpa
NPC6	-	Picea sitchensis
NPC6	-	Arabidopsis thaliana
PC-PLC	-	Pseudomonas fluorescens
PC-PLC	-	Bacillus cereus
PC-PLC	-	Clostridium perfringens
PC-PLC	-	Ureaplasma urealyticum
phosphatidylcholine-specific phospholipase C	-	Pseudomonas fluorescens
phosphatidylcholine-specific phospholipase C	-	Bacillus cereus
phosphatidylcholine-specific phospholipase C	-	Clostridium perfringens
phosphatidylcholine-specific phospholipase C	-	Ureaplasma urealyticum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	broad pH optimum	Ureaplasma urealyticum

Expression

Organism	Comment	Expression
Arabidopsis thaliana	the enzyme is induced by 24-epibrassinolide signalling, auxin, and cytokinin. Expression level of NPC3 is increased 14.6fold after 2 h in seedlings subjected to 37°C heat stress. NPC3 does demonstrate a positive response to Botrytis cinerea, Golovinomyces orontii, Pseudomonas syringae and Phytophthora infestans treatment	up
Arabidopsis thaliana	the enzyme is induced by phosphate deficiency	up

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Nicotiana tabacum
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Glycine max
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Sorghum bicolor
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Oryza sativa
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Petunia x hybrida
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Physcomitrium patens
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Vitis vinifera
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Populus trichocarpa
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Picea sitchensis
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Selaginella moellendorffii
evolution	the enzyme belongs to the plant non-specific phospholipase C gene family, phylogenetic tree, overview. The common ancestor of all seed plants already had at least one NPC1-, NPC2- and NPC6-like gene. Non-specific phospholipases C are a distinct type of plant phospholipid-cleaving enzyme homologous to bacterial phosphatidylcholine-specific phospholipases C	Arabidopsis thaliana
malfunction	npc4 knockout mutants are characterised by a reduced germination rate when sown on media containing 150 mM NaCl. Mutant npc4 plants also have reduced germination and overall viability under salt and drought stress conditions. Unlike wild-type plants, mutants overexpressing NPC4 are characterised by a higher germination level and maintain a greater root length and dry weight under both salt stress and hyperosmosis	Arabidopsis thaliana
malfunction	the N-terminal domain of a-toxin retains PC-PLC activity when expressed in Escherichia coli, but lacks haemolytic and sphingomyelinase activities that are supposedly granted by a lipoxygenase-like C-terminal domain	Clostridium perfringens
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Nicotiana tabacum
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Glycine max
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Sorghum bicolor
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Oryza sativa
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Petunia x hybrida
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Physcomitrium patens
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Vitis vinifera
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Populus trichocarpa
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Picea sitchensis
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Selaginella moellendorffii
metabolism	model of metabolism regulation carried out by plant cell phospholipases, overview	Arabidopsis thaliana
additional information	sequence comparisons and three-dimensional structure modeling, overview	Nicotiana tabacum
additional information	sequence comparisons and three-dimensional structure modeling, overview	Glycine max
additional information	sequence comparisons and three-dimensional structure modeling, overview	Sorghum bicolor
additional information	sequence comparisons and three-dimensional structure modeling, overview	Oryza sativa
additional information	sequence comparisons and three-dimensional structure modeling, overview	Petunia x hybrida
additional information	sequence comparisons and three-dimensional structure modeling, overview	Physcomitrium patens
additional information	sequence comparisons and three-dimensional structure modeling, overview	Vitis vinifera
additional information	sequence comparisons and three-dimensional structure modeling, overview	Populus trichocarpa
additional information	sequence comparisons and three-dimensional structure modeling, overview	Picea sitchensis
additional information	sequence comparisons and three-dimensional structure modeling, overview	Selaginella moellendorffii
additional information	sequence comparisons and three-dimensional structure modeling, overview	Arabidopsis thaliana
additional information	the N-terminal domain contains the phospholipase C active site, which also incorporates zinc ions. The C-terminal C2-like PLAT (polycystin-1, lipoxygenase, alpha-toxin) domain was found to be similar to lipid binding domains in eukaryotes and appears to be responsible for binding membrane phospholipids in a calcium-dependent manner	Clostridium perfringens
physiological function	NPC3 might play a rolei in thermotolerance. The enzyme is responsible for lipid conversion during phosphate-limiting conditions. Two articles non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview, inducible expression and putative signalling role	Arabidopsis thaliana
physiological function	NPC4 participates in triggering plant salt stress responses likely via abscisic acid-dependent mechanisms. The enzyme is responsible for lipid conversion during phosphate-limiting conditions. Two articles non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview, inducible expression and putative signalling role	Arabidopsis thaliana
physiological function	the enzyme inhibits the formation of cAMP by adenylate cyclase and is involved in the defence mechanism of bacteria to phagocytosis	Bacillus cereus
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Glycine max
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Sorghum bicolor
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Oryza sativa
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Petunia x hybrida
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Physcomitrium patens
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Vitis vinifera
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Populus trichocarpa
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Picea sitchensis
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Selaginella moellendorffii
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Two articles non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Nicotiana tabacum
physiological function	the enzyme is responsible for lipid conversion during phosphate-limiting conditions. Two articles non-specific phospholipases C are involved in biotic and abiotic stress responses as well as phytohormone actions. The diacylglycerol produced via the enzymes is postulated to participate in membrane remodelling, general lipid metabolism and cross-talk with other phospholipid signalling systems in plants. Mode of action of the enzyme in lipid metabolism, signal transduction, and membrane remodelling, detailed overview	Arabidopsis thaliana
physiological function	the secreted enzyme plays a role in the aggregation of blood platelets and inhibits defensive superoxide generation in human polymorphonuclear leukocytes by interacting with membrane components of NADPH oxidase	Clostridium perfringens