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Literature summary for 3.1.4.3 extracted from

  • Mo, S.; Kim, J.H.; Cho, K.W.
    A novel extracellular phospholipase C purified from a marine bacterium, Pseudoalteromonas sp. J937 (2009), Biotechnol. Lett., 31, 89-94.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ activity is enhanced by 150% with Ca2+ (200 mM) Pseudoalteromonas sp.
Na+ activity is enhanced by 180% with Na+ (500 mM) Pseudoalteromonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudoalteromonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp.
-
 J937
-
Pseudoalteromonas sp. J937
-
 J937
-

Purification (Commentary)

Purification (Comment) Organism
by serial ion exchange and gel filtration column chromatography Pseudoalteromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of 33 U/mg protein Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids Pseudoalteromonas sp. ?
-
 ?
additional information phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids Pseudoalteromonas sp. J937 ?
-
 ?
phosphatidylcholine + H2O
-
 Pseudoalteromonas sp. 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O
-
 Pseudoalteromonas sp. J937 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylethanolamine + H2O
-
 Pseudoalteromonas sp. phosphorylethanolamine + 1,2-diacylglycerol
-
 ?
phosphatidylethanolamine + H2O
-
 Pseudoalteromonas sp. J937 phosphorylethanolamine + 1,2-diacylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
phospholipase C
-
 Pseudoalteromonas sp.
PLC
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudoalteromonas sp.