Literature summary for 3.1.4.3 extracted from

Stonehouse, M.J.; Cota-Gomez, A.; Parker, S.K.; Martin, W.E.; Hankin, J.A.; Murphy, R.C.; Chen, W.; Lim, K.B.; Hackett, M.; Vasil, A.I.; Vasil, M.L.
A novel class of microbial phosphocholine-specific phospholipases C (2002), Mol. Microbiol., 46, 661-676.

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Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition of enzymatic activity by Ca2+, inhibits haemolytic activity of the enzyme	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
14.1	-	p-Nitrophenylphosphorylcholine	37°C, pH 7.2	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17000	-	1 * 79000 + 1 * 17000, SDS-PAGE	Pseudomonas aeruginosa
79000	-	1 * 79000 + 1 * 17000, SDS-PAGE	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
149.7	-	-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenylphosphorylcholine + H2O	-	Pseudomonas aeruginosa	p-nitrophenol + phosphorylcholine	-	?
phosphatidylcholine + H2O	-	Pseudomonas aeruginosa	1,2-diacylglycerol + phosphorylcholine	-	?
plasmalogen + H2O	-	Pseudomonas aeruginosa	?	-	?
sphingomyelin + H2O	-	Pseudomonas aeruginosa	ceramide + phosphorylcholine	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 79000 + 1 * 17000, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
PlcHR2	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
979	-	p-Nitrophenylphosphorylcholine	37°C, PH 7.2	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)