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Literature summary for 3.1.4.3 extracted from

  • Dubouix, A.; Nieto, M.; Fauvel, J.; Chap, H.; Marty, N.; Salles, J.P.; Gaits, F.
    A simple and reliable method for rapid production and purification of Pseudomonas aeruginosa haemolytic phospholipase C (2004), Lett. Appl. Microbiol., 38, 191-196.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
Zn2+ required, 10 mM Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
production of the recombinant protein in Escherichia coli is no more successful because of frequent inclusion bodies and accumulation of inactive phospholipase C in the periplasmic space Pseudomonas aeruginosa

General Stability

General Stability Organism
CHAPS, 20 mM, or Triton X-100, 20 mM, no loss of activity Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information the recombinant enzyme is resistant to 20 mM CHAPS or 20 mM Triton X-100 Pseudomonas aeruginosa
Zn2+ recombinant enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72800
-
 x * 72800, truncated recombinant protein with His6-tag, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa
-
 recombinant enzyme, production in Schizosaccharomyces pombe
-

Purification (Commentary)

Purification (Comment) Organism
method for production of recombinant enzyme in Schizosaccharomyces pombe Pseudomonas aeruginosa
recombinant Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
-
 Pseudomonas aeruginosa
1940
-
 pH 7.3, 37°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenylphosphorylcholine + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O substrate from egg yolk Pseudomonas aeruginosa 1,2-diacylglycerol + phosphorylcholine
-
 ?

Subunits

Subunits Comment Organism
? x * 72800, truncated recombinant protein with His6-tag, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
haemolytic phospholipase C
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 1 min, complete loss of activity Pseudomonas aeruginosa