Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, 10 mM | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
production of the recombinant protein in Escherichia coli is no more successful because of frequent inclusion bodies and accumulation of inactive phospholipase C in the periplasmic space | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
CHAPS, 20 mM, or Triton X-100, 20 mM, no loss of activity | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the recombinant enzyme is resistant to 20 mM CHAPS or 20 mM Triton X-100 | Pseudomonas aeruginosa | |
Zn2+ | recombinant enzyme | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72800 | - |
x * 72800, truncated recombinant protein with His6-tag, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | - |
recombinant enzyme, production in Schizosaccharomyces pombe | - |
Purification (Comment) | Organism |
---|---|
method for production of recombinant enzyme in Schizosaccharomyces pombe | Pseudomonas aeruginosa |
recombinant | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas aeruginosa |
1940 | - |
pH 7.3, 37°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenylphosphorylcholine + H2O | - |
Pseudomonas aeruginosa | p-nitrophenol + phosphorylcholine | - |
? | |
phosphatidylcholine + H2O | substrate from egg yolk | Pseudomonas aeruginosa | 1,2-diacylglycerol + phosphorylcholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72800, truncated recombinant protein with His6-tag, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
haemolytic phospholipase C | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
1 min, complete loss of activity | Pseudomonas aeruginosa |