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Literature summary for 3.1.4.3 extracted from

  • Martin, S.F.; Follows, B.C.; Hergenrother, P.J.; Trotter, B.K.
    The choline binding site of phospholipase C (Bacillus cereus): insights into substrate specificity (2000), Biochemistry, 39, 3410-3415.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
Zn2+ required, three ions per active site Bacillus cereus

Protein Variants

Protein Variants Comment Organism
E4D significant decrease in catalytic efficiency Bacillus cereus
E4L significant decrease in catalytic efficiency Bacillus cereus
E4Q significant decrease in catalytic efficiency Bacillus cereus
F66A dramatic decrease in catalytic efficiency Bacillus cereus
F66R dramatic decrease in catalytic efficiency Bacillus cereus
F66W catalytic activity comparable to wild type Bacillus cereus
Y56A little decrease in catalytic efficiency Bacillus cereus
Y56R little decrease in catalytic efficiency Bacillus cereus
Y56W little decrease in catalytic efficiency Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine pH 7.3 Bacillus cereus
2.4
-
 1,2-dihexanoyl-sn-glycero-3-phosphocholine pH 7.3 Bacillus cereus
4.5
-
 1,2-dihexanoyl-sn-glycero-3-phospho-L-serine pH 7.3 Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
 x * 28500, deduced from gene sequence Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus P09598
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihexanoyl-sn-glycero-3-phospho-L-serine + H2O
-
 Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphorylserine
-
 ?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
 Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphorylcholine
-
 ?
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O
-
 Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
-
 ?

Subunits

Subunits Comment Organism
? x * 28500, deduced from gene sequence Bacillus cereus