Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.3 extracted from

  • Otnaess, A.B.; Little, C.; Sletten, K.; Wallin, R.; Johnsen, S.; Flengsrud, R.; Prydz, H.
    Some characteristics of phospholipase C from Bacillus cereus (1977), Eur. J. Biochem., 79, 459-468.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate 0.1% Bacillus cereus

Application

Application Comment Organism
medicine inactivation of thromboplastin, potential therapeutic value in cases of intravascular coagulation Bacillus cereus

General Stability

General Stability Organism
1,10-phenanthroline, denaturation Bacillus cereus
guanidine HCl, stable Bacillus cereus
sodium dodecyl sulfate Bacillus cereus
sodium dodecyl sulfate, denaturation Bacillus cereus
sodium dodecyl sulfate, enzyme resistant to denaturation in presence of 1 mM Zn2+ Bacillus cereus
urea, enzyme stable in presence of 1 mM Zn2+ Bacillus cereus
urea, PLC is catalytically active in 8 M urea Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Bacillus cereus
o-phenanthroline denaturation Bacillus cereus
SDS
-
 Bacillus cereus
Urea denaturation; denaturation only in absence of free Zn2+ Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 1,2-dipalmitoylphosphatidylcholine
-
 Bacillus cereus
2 3 phosphatidylserine
-
 Bacillus cereus
4.5 7 1,2-dipalmitoylphosphatidylethanolamine
-
 Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloenzyme Bacillus cereus
Zn2+ 2 mol Zn2+ per mol enzyme Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
-
 Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
 strain ATCC 10987 AB-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Storage Stability

Storage Stability Organism
-20°C, in barbital-buffered saline, pH 7.4, 1 mM ZnSO4, stable for more than one year Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dicaproylphosphatidylcholine + H2O
-
 Bacillus cereus 1,2-dicaproylglycerol + phosphorylcholine
-
 ?
1,2-dimyristoylphosphatidylcholine + H2O
-
 Bacillus cereus 1,2-dimyristoylglycerol + phosphorylcholine
-
 ?
1,2-dipalmitoylphosphatidylcholine + H2O
-
 Bacillus cereus 1,2-dipalmitoylglycerol + phosphorylcholine
-
 ?
1,2-dipalmitoylphosphatidylethanolamine + H2O
-
 Bacillus cereus 1,2-dipalmitoylglycerol + phosphorylethanolamine
-
 ?
phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylethanolamine + H2O
-
 Bacillus cereus phosphorylethanolamine + diacylglycerol
-
 ?
phosphatidylserine + H2O
-
 Bacillus cereus phosphorylserine + diacylglycerol
-
 ?

Subunits

Subunits Comment Organism
monomer 23000, no comment on method Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 60 substrate: dimyristoylphosphatidylcholine Bacillus cereus
47
-
 substrate: dipalmitoylphosphatidylcholine Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 76
-
 Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
76
-
 for 6 min, with 0.1 mM free Zn2+: loss of 21% of activity, without Zn2+: loss of 63% of activity Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate: dicaproyl phosphatidylcholine Bacillus cereus
8 8.3 subatrate: dipalmitoylphosphatidylcholine Bacillus cereus