Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.3 extracted from

  • Little, C.; Johansen, S.
    Unfolding and refolding of phospholipase C from Bacillus cereus in solutions of guanidinium chloride (1979), Biochem. J., 179, 509-514.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.3

General Stability

General Stability Organism
guanidinium chloride, denaturation at a concentration of 1-2 M, reversible by free Zn2+ Bacillus cereus
urea, PLC is catalytically active in 8 M urea Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Bacillus cereus
Guanidinium chloride denaturation Bacillus cereus
Urea denaturation Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloenzyme Bacillus cereus
Zn2+ 2 mol Zn2+ per mol enzyme Bacillus cereus
Zn2+ renaturation of guanidinium chloride treated PLC Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
-
 Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
guanidinium chloride causes denaturation at a concentration of 1-2 M, reversible by free Zn2+ Bacillus cereus

Subunits

Subunits Comment Organism
monomer 23000, no comment on method Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 for 5 min, in 2 M guanidinium chloride, no loss of activity after dialysis against Zn2+-containing buffer Bacillus cereus