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Literature summary for 3.1.4.3 extracted from
- A phospholipase C fron the Dallas IE strain of Legionella pneumophila serogroup. V. Purification and characterization of conditions for optimal activity with an artificial substrate (1988), J. Gen. Microbiol., 134, 489-498.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sorbitol | - |
Legionella pneumophila |  |
| Triton X-100 | - |
Legionella pneumophila | |
| Tween 20 | - |
Legionella pneumophila | |
| Tween 80 | - |
Legionella pneumophila | |
General Stability
| General Stability | Organism |
|---|---|
| sodium dodecyl sulfate | Legionella pneumophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Legionella pneumophila | |
| EDTA | - |
Legionella pneumophila | |
| Fe2+ | - |
Legionella pneumophila | |
| SDS | - |
Legionella pneumophila | |
| Zn2+ | - |
Legionella pneumophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | 61 | p-Nitrophenylphosphorylcholine | - |
Legionella pneumophila | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Legionella pneumophila | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | restores activity after EDTA treatment | Legionella pneumophila | |
| Ca2+ | restores activity after EDTA treatment | Legionella pneumophila | |
| Co2+ | restores activity after EDTA treatment | Legionella pneumophila | |
| Mg2+ | restores activity after EDTA treatment | Legionella pneumophila | |
| Mn2+ | restores activity after EDTA treatment | Legionella pneumophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | 54000 | SDS-PAGE | Legionella pneumophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Legionella pneumophila | - |
1,2-diacylglycerol + phosphorylcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Legionella pneumophila | - |
Dallas 1E strain, serogroup 5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography, fractionation by manganous chloride, ammonium sulfate precipitation steps | Legionella pneumophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.029 | - |
substrate: p-nitrophenylphosphorylcholine | Legionella pneumophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dipalmitoylphosphatidylcholine + H2O | - |
Legionella pneumophila | 1,2-dipalmitoylglycerol + phosphorylcholine | - |
? | |
| p-nitrophenylphosphorylcholine + H2O | - |
Legionella pneumophila | p-nitrophenol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Legionella pneumophila | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
substrate: p-nitrophenylphosphorylcholine | Legionella pneumophila |
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