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Literature summary for 3.1.4.3 extracted from
- Purification and characterization of phospholipase C preferentially hydrolysing phosphatidylcholine in Tetrahymena membranes (1993), J. Eukaryot. Microbiol., 40, 775-781.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Nonidet P-40 | - |
Tetrahymena pyriformis |  |
| sodium deoxycholate | 0.05% | Tetrahymena pyriformis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Tetrahymena pyriformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.166 | - |
1,2-dipalmitoylphosphatidylcholine | - |
Tetrahymena pyriformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Tetrahymena pyriformis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Tetrahymena pyriformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 127000 | - |
gel filtration | Tetrahymena pyriformis |
| 128000 | - |
SDS-PAGE | Tetrahymena pyriformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena pyriformis | - |
- |
- |
| Tetrahymena pyriformis W | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| column chromatography, gel filtration chromatography, 190fold | Tetrahymena pyriformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.083 | - |
substrate: dipalmitoylphosphatidylcholine | Tetrahymena pyriformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dioleoylphosphatidylethanolamine + H2O | - |
Tetrahymena pyriformis | 1,2-dioleoylglycerol + phosphorylethanolamine | - |
? | |
| 1,2-dioleoylphosphatidylethanolamine + H2O | - |
Tetrahymena pyriformis W | 1,2-dioleoylglycerol + phosphorylethanolamine | - |
? | |
| 1,2-dipalmitoylphosphatidylcholine + H2O | - |
Tetrahymena pyriformis | 1,2-dipalmitoylglycerol + phosphorylcholine | - |
? | |
| 1,2-dipalmitoylphosphatidylcholine + H2O | - |
Tetrahymena pyriformis W | 1,2-dipalmitoylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Tetrahymena pyriformis | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Tetrahymena pyriformis W | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylethanolamine + H2O | - |
Tetrahymena pyriformis | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylethanolamine + H2O | - |
Tetrahymena pyriformis W | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylinositol + H2O | - |
Tetrahymena pyriformis | inositol monophosphate + diacylglycerol | - |
? | |
| phosphatidylinositol + H2O | - |
Tetrahymena pyriformis W | inositol monophosphate + diacylglycerol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 127000-128000, gel filtration, SDS-PAGE | Tetrahymena pyriformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
substrate: dipalmitoylphosphatidylcholine | Tetrahymena pyriformis |
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