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Literature summary for 3.1.4.3 extracted from

  • Meij, J.T.A.; Ross, E.T.
    Purification and characterization of phospholipase C-¯1 mutants expressed in E.coli (1996), Biochem. Biophys. Res. Commun., 225, 705-711.
    View publication on PubMed
Search for more literature for 3.1.4.3

Activating Compound

Activating Compound Comment Organism Structure
subunit of cell surface receptor associated G-protein Gqa, subunit of cell surface receptor associated G-protein, PLC d1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
tranformed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
G758D
-
 Homo sapiens
S541Y
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 PLC beta 1, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylinositol-bisphosphate + H2O Homo sapiens
-
 inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PLC, NiNTA, two FPLC steps, 160fold Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1050000
-
 substrate: phosphatidylinositol-4,5-bisphosphate Homo sapiens

Storage Stability

Storage Stability Organism
-80°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol-bisphosphate + H2O
-
 Homo sapiens inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?