Cloned (Comment) | Organism |
---|---|
transformed into Escherichia coli | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
E4A | Kunkel method | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphatidic acid | monomeric and micellar, poor substrate, inhibitor | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
diheptanoylphosphatidylcholine | micellar substrate, wild-type PLC | Bacillus cereus | |
0.19 | - |
dihexanoylphosphatidylcholine | monomeric substrate, wild-type PLC | Bacillus cereus | |
1.09 | - |
diheptanoylphosphatidylcholine | micellar substrate, recombinant PLC | Bacillus cereus | |
1.12 | - |
dihexanoylphosphatidylcholine | monomeric substrate, recombinant PLC | Bacillus cereus | |
4.2 | - |
dihexanoylphosphatidic acid | monomeric substrate, recombinant PLC | Bacillus cereus | |
9 | - |
dihexanoylphosphatidic acid | monomeric substrate, wild-type PLC | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloenzyme | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28500 | - |
- |
Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diheptanoylphosphatidylcholine + H2O | - |
Bacillus cereus | diheptanoylglycerol + phosphorylcholine | - |
? | |
dihexanoylphosphatidic acid + H2O | - |
Bacillus cereus | ? | - |
? | |
dihexanoylphosphatidylcholine + H2O | - |
Bacillus cereus | dihexanoylglycerol + phosphorylcholine | - |
? | |
phosphatidic acid + H2O | - |
Bacillus cereus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
dihexanoylphosphatidic acid | monomeric substrate, wild-type PLC | Bacillus cereus | |
0.0198 | - |
dihexanoylphosphatidic acid | monomeric substrate, recombinant PLC | Bacillus cereus | |
4.93 | - |
dihexanoylphosphatidylcholine | monomeric substrate, recombinant PLC | Bacillus cereus | |
6.78 | - |
dihexanoylphosphatidylcholine | monomeric substrate, wild-type PLC | Bacillus cereus | |
6.85 | - |
diheptanoylphosphatidylcholine | micellar substrate, recombinant PLC | Bacillus cereus | |
13.8 | - |
diheptanoylphosphatidylcholine | micellar substrate, wild-type PLC | Bacillus cereus |