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Literature summary for 3.1.4.3 extracted from

  • Tan, C.A.; Roberts, M.F.
    Engineering of the nonspecific phospholipase C from Bacillus cereus: Replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity (1998), Biochemistry, 37, 4275-4279.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
transformed into Escherichia coli Bacillus cereus

Protein Variants

Protein Variants Comment Organism
E4A Kunkel method Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
phosphatidic acid monomeric and micellar, poor substrate, inhibitor Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
diheptanoylphosphatidylcholine micellar substrate, wild-type PLC Bacillus cereus
0.19
-
dihexanoylphosphatidylcholine monomeric substrate, wild-type PLC Bacillus cereus
1.09
-
diheptanoylphosphatidylcholine micellar substrate, recombinant PLC Bacillus cereus
1.12
-
dihexanoylphosphatidylcholine monomeric substrate, recombinant PLC Bacillus cereus
4.2
-
dihexanoylphosphatidic acid monomeric substrate, recombinant PLC Bacillus cereus
9
-
dihexanoylphosphatidic acid monomeric substrate, wild-type PLC Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloenzyme Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
-
Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diheptanoylphosphatidylcholine + H2O
-
Bacillus cereus diheptanoylglycerol + phosphorylcholine
-
?
dihexanoylphosphatidic acid + H2O
-
Bacillus cereus ?
-
?
dihexanoylphosphatidylcholine + H2O
-
Bacillus cereus dihexanoylglycerol + phosphorylcholine
-
?
phosphatidic acid + H2O
-
Bacillus cereus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
dihexanoylphosphatidic acid monomeric substrate, wild-type PLC Bacillus cereus
0.0198
-
dihexanoylphosphatidic acid monomeric substrate, recombinant PLC Bacillus cereus
4.93
-
dihexanoylphosphatidylcholine monomeric substrate, recombinant PLC Bacillus cereus
6.78
-
dihexanoylphosphatidylcholine monomeric substrate, wild-type PLC Bacillus cereus
6.85
-
diheptanoylphosphatidylcholine micellar substrate, recombinant PLC Bacillus cereus
13.8
-
diheptanoylphosphatidylcholine micellar substrate, wild-type PLC Bacillus cereus