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Literature summary for 3.1.4.2 extracted from

  • Morita, J.; Kano, K.; Kato, K.; Takita, H.; Sakagami, H.; Yamamoto, Y.; Mihara, E.; Ueda, H.; Sato, T.; Tokuyama, H.; Arai, H.; Asou, H.; Takagi, J.; Ishitani, R.; Nishimasu, H.; Nureki, O.; Aoki, J.
    Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase (2016), Sci. Rep., 6, 20995 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
C393A/C412S mutant apoenzyme in complex with sn-glycero-3-phosphocholine, vapor diffusion method, using 0.2 M NH4Cl, 0.1 M sodium acetate pH 5.0, 20% (w/v) PEG 6000 Mus musculus

Protein Variants

Protein Variants Comment Organism
C154A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
C393A/C412S the mutations do not affect enzyme activity Mus musculus
E143A the mutant enzyme shows about 50% reduced activity compared to the wild type enzyme Mus musculus
E190A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
S71A the mutant enzyme is almost inactive Mus musculus
S71T inactive Mus musculus
Y188A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
Y72A the mutant enzyme is almost inactive Mus musculus
Y75A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
Y75A/Y188A inactive Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Mus musculus 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the enzyme contains 2 Zn2+ ions Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycero-3-phosphocholine + H2O Mus musculus specific substrate choline + sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8BGN3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
heart
-
 Mus musculus
-
kidney
-
 Mus musculus
-
liver sinusoidal endothelial cell
-
 Mus musculus
-
lung
-
 Mus musculus
-
oligodendrocyte developing oligodendrocytes Mus musculus
-
spleen
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycero-3-phosphocholine + H2O
-
 Mus musculus choline + sn-glycerol 3-phosphate
-
 ?
sn-glycero-3-phosphocholine + H2O specific substrate Mus musculus choline + sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
choline-specific glycerophosphodiester-phosphodiesterase
-
 Mus musculus
ENPP6
-
 Mus musculus

General Information

General Information Comment Organism
malfunction enzyme-deficient mice exhibit fatty liver and hypomyelination Mus musculus
metabolism the enzyme participates in choline metabolism Mus musculus