Cloned (Comment) | Organism |
---|---|
genes glpQ and mpn566, expression of N-terminally Strep-tagged enzymes | Mycoplasma pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generationof GlpQ and of MPN566 knockout strains, phenotypes, overview. The MPN566-deficient strain does not show an altered phenotype | Mycoplasma pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activity with | Mycoplasma pneumoniae | |
Co2+ | no activity with | Mycoplasma pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, required for activity | Mycoplasma pneumoniae | |
Mn2+ | activates, less activating than Mg2+ | Mycoplasma pneumoniae | |
Zn2+ | activates, less activating than Mg2+ | Mycoplasma pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycero-3-phosphocholine + H2O | Mycoplasma pneumoniae | - |
choline + sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma pneumoniae | - |
gene glpQ | - |
Purification (Comment) | Organism |
---|---|
N-terminally Strep-tagged enzymes | Mycoplasma pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycero-3-phosphocholine + H2O | - |
Mycoplasma pneumoniae | choline + sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlpQ | - |
Mycoplasma pneumoniae |
glycerophosphodiesterase | - |
Mycoplasma pneumoniae |
MPN420 | - |
Mycoplasma pneumoniae |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of the glpQ gene results in reduced growth in medium with glucose as the carbon source, in loss of hydrogen peroxide production when phosphatidylcholine is present, and in a complete loss of cytotoxicity towards HeLa cells, and the glpQ mutant strain exhibited a reduced gliding velocity, overview. The phenotype is reversible par complementation with the wild-type enzyme | Mycoplasma pneumoniae |
additional information | a second glycerophosphodiesterase enzyme, MPN566, is catalytically inactive and its deletion does not cause an altered phenotype | Mycoplasma pneumoniae |
physiological function | GlpQ is implicated in the control of gene expression, apparent regulation by GlpQ is exerted at the level of transcription. All genes subject to GlpQ-dependent control have a conserved potential cis-acting element upstream of the coding region. This element overlaps the promoter in the case of the genes that are repressed in a GlpQ-dependent manner and it is located upstream of the promoter for GlpQ-activated genes. GlpQ is central to the normal physiology and to pathogenicity of the minimal pathogen | Mycoplasma pneumoniae |