Cloned (Comment) | Organism |
---|---|
expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | GDE5 RNAi gene silencing results in an increase in intracellular sn-glycero-3-phosphocholine levels. Construction of a truncated GDE5 construct, GDE5DELTAC471, which contains a GP-PDE sequence identified in other GP-PDEs but lacks GroPCho phosphodiesterase activity, and it shows a similar inhibitory effect on myogenic differentiation like the full-length enzyme, overview | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, required | Mus musculus | |
additional information | no activation by Ca2+ | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
x * 81000, recombinant His6-tagged enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycero-3-phosphocholine + H2O | Mus musculus | - |
choline + sn-glycerol 3-phosphate | - |
? | |
sn-glycero-3-phosphocholine + H2O | Mus musculus C57BL/6 | - |
choline + sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus C57BL/6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
purified recombinant His-tagged GDE5, substrate is glycerophosphoethanolamine, pH 7.4, 37°C | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerophosphoethanolamine + H2O | - |
Mus musculus | ? | - |
? | |
glycerophosphoethanolamine + H2O | - |
Mus musculus C57BL/6 | ? | - |
? | |
additional information | the enzyme shows no significant activity with glycerophosphoglycerol, GroPIns, and glycerophosphoserine as substrates | Mus musculus | ? | - |
? | |
additional information | the enzyme shows no significant activity with glycerophosphoglycerol, GroPIns, and glycerophosphoserine as substrates | Mus musculus C57BL/6 | ? | - |
? | |
sn-glycero-3-phosphocholine + H2O | - |
Mus musculus | choline + sn-glycerol 3-phosphate | - |
? | |
sn-glycero-3-phosphocholine + H2O | - |
Mus musculus C57BL/6 | choline + sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 81000, recombinant His6-tagged enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
GDE5 | - |
Mus musculus |
glycerophosphodiester phosphodiesterase | - |
Mus musculus |
GP-PDE | - |
Mus musculus |
More | GDE5 is an UgpQ-type GDE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | GDE5 expression is reduced in atrophied skeletal muscles in mice and decreasing GDE5 abundance promotes myoblastic differentiation, suggesting that decreased GDE5 expression has a counter-regulatory effect on the progression of skeletal muscleatrophy. Transgenic mice specifically overexpressing GDE5DELTAC471 in skeletal muscle have less type II fiber-rich skeletal muscle mass. Forced expression of full-length GDE5 in cultured myoblasts suppresses myogenic differentiation. Transgenic mice specifically expressing GDE5DELTAC471 in skeletal muscle show less skeletal muscle mass, especially type II fiber-rich muscle | Mus musculus |
physiological function | GDE5 selectively hydrolyzes glycerophosphocholine and controls skeletal muscle development. GDE5 negatively regulates skeletal muscle development even without GroPCho phosphodiesterase activity | Mus musculus |