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Literature summary for 3.1.4.2 extracted from
- The structure and function of a novel glycerophosphodiesterase from Enterobacter aerogenes (2007), J. Mol. Biol., 367, 1047-1062.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Klebsiella aerogenes |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Klebsiella aerogenes |  |
| Zn2+ | activity of enzyme with Zn2+ 30 fold less than with Co2+ with substrate bis(p-nitro-phenyl)phosphate | Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30800 | - |
monomer | Klebsiella aerogenes |
| 185700 | - |
electrospray ionisation mass spectrometry, under non-denaturing conditions, consistent with calculated mass of hexamer | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycero-3-phosphoethanolamine + H2O | Klebsiella aerogenes | hydrolysis of the 3'-5' phosphodiester bond | sn-glycerol-3-phosphate + ethanolamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis(p-nitro-phenyl)phosphate + H2O | - |
Klebsiella aerogenes | ? | - |
? | |
| glycero-3-phosphoethanolamine + H2O | hydrolysis of the 3'-5' phosphodiester bond | Klebsiella aerogenes | sn-glycerol-3-phosphate + ethanolamine | - |
? | |
| glycero-3-phosphoethanolamine + H2O | also active on phosphomonoester, phosphodiester, phosphotriester and phosphorothiolate substrates | Klebsiella aerogenes | sn-glycerol-3-phosphate + ethanolamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | three dimers stabilized by an inter-chain disulfide bond, alpha/beta-sandwich | Klebsiella aerogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycerophosphodiesterase | - |
Klebsiella aerogenes |
| GpdQ | - |
Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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