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Literature summary for 3.1.4.17 extracted from

  • Zheng, Z.; Zhu, M.; He, Y.; Li, N.; Guo, T.; Chen, Y.; Wu, J.; Ying, H.; Xie, J.
    Gene cloning, expression, and characterization of a cyclic nucleotide phosphodiesterase from Arthrobacter sp. CGMCC 3584 (2013), Appl. Biochem. Biotechnol., 169, 2442-2456.
    View publication on PubMed
Search for more literature for 3.1.4.17

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 2 mM, 28% residual activity Arthrobacter sp.
ADP 1 mM, 56% residual activity Arthrobacter sp.
AMP 1 mM, 15% residual activity Arthrobacter sp.
ATP 1 mM, 46% residual activity Arthrobacter sp.
Ca2+ 2 mM, 69% residual activity Arthrobacter sp.
Cu2+ 2 mM, 27% residual activity Arthrobacter sp.
dipyridamole 1 mM, 71% residual activity Arthrobacter sp.
EDTA 2 mM, 44% residual activity Arthrobacter sp.
GDP 1 mM, 41% residual activity Arthrobacter sp.
GMP 1 mM, 31% residual activity Arthrobacter sp.
GTP 1 mM, 44% residual activity Arthrobacter sp.
NADH 1 mM, 76% residual activity Arthrobacter sp.
NADPH 1 mM, 25% residual activity Arthrobacter sp.
NaF 1 mM, 79% residual activity Arthrobacter sp.
theophylline 1 mM, 70% residual activity Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.82
-
 3',5'-cAMP pH 7.5, 30°C Arthrobacter sp.
12.82
-
 3',5'-cGMP pH 7.5, 30°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2 mM, 212% of initial activity Arthrobacter sp.
Fe3+ 2 mM, 121% of initial activity Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33600
-
 1 * 33600, calculated, 1 * 38000, SDS-PAGE of recombinant protein including His-tag Arthrobacter sp.
37800
-
 gel filtration Arthrobacter sp.
38000
-
 1 * 33600, calculated, 1 * 38000, SDS-PAGE of recombinant protein including His-tag Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. M9W6G9
-
-
Arthrobacter sp. CGMCC 3584 M9W6G9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
 Arthrobacter sp. 5'-AMP
-
 ?
2',3'-cAMP + H2O
-
 Arthrobacter sp. CGMCC 3584 5'-AMP
-
 ?
3',5'-cAMP + H2O
-
 Arthrobacter sp. 5'-AMP
-
 ?
3',5'-cAMP + H2O
-
 Arthrobacter sp. CGMCC 3584 5'-AMP
-
 ?
3',5'-cGMP + H2O
-
 Arthrobacter sp. 5'-GMP
-
 ?
3',5'-cGMP + H2O
-
 Arthrobacter sp. CGMCC 3584 5'-GMP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33600, calculated, 1 * 38000, SDS-PAGE of recombinant protein including His-tag Arthrobacter sp.

Synonyms

Synonyms Comment Organism
CpdA
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8 more than 80% of maximum activiy Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp. calculated
-
 5.5