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Literature summary for 3.1.4.17 extracted from

  • Kimura, Y.; Yoshimi, M.; Takata, G.
    Enzymatic and mutational analyses of a class II 3,5-cyclic nucleotide phosphodiesterase, PdeE, from Myxococcus xanthus (2011), J. Bacteriol., 193, 2053-2057.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.17

Cloned(Commentary)

Cloned (Comment) Organism
gene pdeE, sequence comparisons Myxococcus xanthus

Protein Variants

Protein Variants Comment Organism
additional information construction of a pdeE disruption mutant, phenotype, overview Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ strong inhibition at 1 mM Myxococcus xanthus
additional information the broad-spectrum phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, IBMX, has no effect on the activity of PdeE at 1 mM. No inhibition by theophylline at 1 mM, beta-glycerophosphate at 0.1 mM, and by orthovanadate Myxococcus xanthus
Zn2+ strong inhibition at 1 mM Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 adenosine 3',5'-cyclic phosphate pH 7.0, 55°C Myxococcus xanthus
0.025
-
 guanosine 3',5'-cyclic phosphate pH 7.0, 55°C Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required, activates Myxococcus xanthus
Mn2+ required, activates Myxococcus xanthus
additional information no effect on enzyme activity by Mg2+ Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27600
-
 x * 27600, about, sequence calculation Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
 gene pdeE
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0207
-
 substrate cGMP, pH 7.0, 55°C Myxococcus xanthus
0.0309
-
 substrate cAMP, pH 7.0, 55°C Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 3',5'-cyclic phosphate + H2O
-
 Myxococcus xanthus adenosine 5'-phosphate
-
 ?
guanosine 3',5'-cyclic phosphate + H2O
-
 Myxococcus xanthus guanosine 5'-phosphate
-
 ?
additional information substrate specificity of PdeE, overview Myxococcus xanthus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27600, about, sequence calculation Myxococcus xanthus

Synonyms

Synonyms Comment Organism
class II 3,5-cyclic nucleotide phosphodiesterase
-
 Myxococcus xanthus
PdeE
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Myxococcus xanthus

General Information

General Information Comment Organism
malfunction pdeE mutant exhibits delays in fruiting body and spore formation compared with the wild type when cultured on starvation medium. It also shows reduced enzyme activity Myxococcus xanthus