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Literature summary for 3.1.4.17 extracted from

  • Kimura, Y.; Okazaki, N.; Takegawa, K.
    Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3',5'- and 2'3'-cAMP phosphodiesterase, and phosphatase activities (2009), FEBS Lett., 583, 443-448.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli pG-Tf2/BL21 cells Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ PdeA and PdeB are inhibited (20-30%) at 0.25 mM Co2+ Myxococcus xanthus
EDTA PdeA and PdeB show 39% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM EDTA Myxococcus xanthus
Mn2+ PdeA and PdeB are inhibited (20-30%) at 0.25 mM Mn2+ Myxococcus xanthus
additional information PdeA and PdeB are not stimulated by 3-isobuthyl-1-methylxanthine, theophylline, beta-glycerophosphate, Ca2+, Mg2+, Fe2+, and Fe3+ Myxococcus xanthus
orthovanadate PdeA shows 26% relative activity and PdeB shows 29% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM orthovanadate Myxococcus xanthus
phosphate PdeA and PdeB enzyme activities are strongly inhibited by 0.1 M sodium phosphate buffer (pH 6.0 and 7.0), the PdeB activity is more strongly inhibited by 0.1 M phosphate than is PdeA activity Myxococcus xanthus
phosphoserine PdeA shows 59% relative activity and PdeB shows 73% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM phosphoserine Myxococcus xanthus
phosphotyrosine PdeA shows 44% relative activity and PdeB shows 52% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM phosphotyrosine Myxococcus xanthus
Zn2+ PdeA and PdeB are potently inhibited in the hydrolysis of 3',5'-cAMP by 0.05 mM Zn2+ with 24% and 28% of remaining activity Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
5'-AMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0016
-
5'-AMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0033
-
3',5'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0033
-
5'-ATP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0038
-
2',3'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.005
-
3',5'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0052
-
2',3'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0125
-
5'-ATP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme activities of PdeA and PdeB in the hydrolysis of 3',5'-cAMP are stimulated 3.2fold and 1.98fold at pH 8.0 in 50 mM Tris-HCl buffer by the addition of Co2+ at 0.05 mM, respectively Myxococcus xanthus
Mn2+ the enzyme activities of PdeA and PdeB in the hydrolysis of 3',5'-cAMP are stimulated 4fold and 2.14fold at pH 8.0 in 50 mM Tris-HCl buffer by the addition of Mn2+ at 0.05 mM, respectively Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
PdeA, SDS-PAGE Myxococcus xanthus
34000
-
PdeB, SDS-PAGE Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon column chromatography Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O in PdeB the phosphodiesterase activity for 2',3'-cAMP is about 1.7fold higher than that for 3',5'-cAMP Myxococcus xanthus 3'-AMP
-
?
3',5'-cAMP + H2O 3',5'-cAMP is completely hydrolyzed to adenosine when incubated with PdeA for a prolonged time Myxococcus xanthus 5'-AMP
-
?
3'-AMP + H2O high activity Myxococcus xanthus ?
-
?
3'-GMP + H2O high activity Myxococcus xanthus ?
-
?
5'-ATP + H2O
-
Myxococcus xanthus ?
-
?
5'-dAMP + H2O low activity Myxococcus xanthus ?
-
?
5'-UMP + H2O high activity Myxococcus xanthus ?
-
?
additional information 3',5'-cGMP is either not hydrolysed or is cleaved very slowly by PdeA and PdeB, PdeA and PdeB show no activities toward 2'-AMP and 2'-GMP Myxococcus xanthus ?
-
?
additional information PdeA and PdeB hydrolyze 3',5'- and 2',3'-cyclic AMP to adenosine, and also demonstrate phosphatase activity toward nucleoside 5'-tri-, 5'-di-, 5'- and 3'-monophosphates with highest activities for nucleoside 5'-monophosphates Myxococcus xanthus ?
-
?
UDP-D-glucose + H2O UDP-D-glucose is hydrolyzed by PdeB, but not by PdeA Myxococcus xanthus ?
-
?

Synonyms

Synonyms Comment Organism
PdeA has 3',5'- and 2',3'-cAMP phosphodiesterase and phosphomonoesterase activities Myxococcus xanthus
PdeB has 3',5'- and 2',3'-cAMP phosphodiesterase and phosphomonoesterase activities Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
the optimum temperature for the 3',5'-cAMP phosphodiesterase activity of PdeA is 40°C Myxococcus xanthus
50
-
the optimum temperature for the 3',5'-cAMP phosphodiesterase activity of PdeA is 50°C Myxococcus xanthus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55
-
Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
2',3'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00093
-
3',5'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00096
-
2',3'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00096
-
3',5'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00155
-
5'-ATP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.0031
-
5'-AMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00454
-
5'-ATP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00551
-
5'-AMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 for 3',5'-cAMP with 0.1 M Tris-HCl buffer Myxococcus xanthus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 PdeB shows no 3',5'-cAMP phosphodiesterase activity at or below pH 6 Myxococcus xanthus