Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli pG-Tf2/BL21 cells | Myxococcus xanthus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | PdeA and PdeB are inhibited (20-30%) at 0.25 mM Co2+ | Myxococcus xanthus | |
EDTA | PdeA and PdeB show 39% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM EDTA | Myxococcus xanthus | |
Mn2+ | PdeA and PdeB are inhibited (20-30%) at 0.25 mM Mn2+ | Myxococcus xanthus | |
additional information | PdeA and PdeB are not stimulated by 3-isobuthyl-1-methylxanthine, theophylline, beta-glycerophosphate, Ca2+, Mg2+, Fe2+, and Fe3+ | Myxococcus xanthus | |
orthovanadate | PdeA shows 26% relative activity and PdeB shows 29% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM orthovanadate | Myxococcus xanthus | |
phosphate | PdeA and PdeB enzyme activities are strongly inhibited by 0.1 M sodium phosphate buffer (pH 6.0 and 7.0), the PdeB activity is more strongly inhibited by 0.1 M phosphate than is PdeA activity | Myxococcus xanthus | |
phosphoserine | PdeA shows 59% relative activity and PdeB shows 73% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM phosphoserine | Myxococcus xanthus | |
phosphotyrosine | PdeA shows 44% relative activity and PdeB shows 52% relative activity in the hydrolysis of 3',5'-cAMP in the presence of 0.05 mM phosphotyrosine | Myxococcus xanthus | |
Zn2+ | PdeA and PdeB are potently inhibited in the hydrolysis of 3',5'-cAMP by 0.05 mM Zn2+ with 24% and 28% of remaining activity | Myxococcus xanthus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
5'-AMP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0016 | - |
5'-AMP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0033 | - |
3',5'-cAMP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0033 | - |
5'-ATP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0038 | - |
2',3'-cAMP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.005 | - |
3',5'-cAMP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0052 | - |
2',3'-cAMP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0125 | - |
5'-ATP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme activities of PdeA and PdeB in the hydrolysis of 3',5'-cAMP are stimulated 3.2fold and 1.98fold at pH 8.0 in 50 mM Tris-HCl buffer by the addition of Co2+ at 0.05 mM, respectively | Myxococcus xanthus | |
Mn2+ | the enzyme activities of PdeA and PdeB in the hydrolysis of 3',5'-cAMP are stimulated 4fold and 2.14fold at pH 8.0 in 50 mM Tris-HCl buffer by the addition of Mn2+ at 0.05 mM, respectively | Myxococcus xanthus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
PdeA, SDS-PAGE | Myxococcus xanthus |
34000 | - |
PdeB, SDS-PAGE | Myxococcus xanthus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon column chromatography | Myxococcus xanthus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | in PdeB the phosphodiesterase activity for 2',3'-cAMP is about 1.7fold higher than that for 3',5'-cAMP | Myxococcus xanthus | 3'-AMP | - |
? | |
3',5'-cAMP + H2O | 3',5'-cAMP is completely hydrolyzed to adenosine when incubated with PdeA for a prolonged time | Myxococcus xanthus | 5'-AMP | - |
? | |
3'-AMP + H2O | high activity | Myxococcus xanthus | ? | - |
? | |
3'-GMP + H2O | high activity | Myxococcus xanthus | ? | - |
? | |
5'-ATP + H2O | - |
Myxococcus xanthus | ? | - |
? | |
5'-dAMP + H2O | low activity | Myxococcus xanthus | ? | - |
? | |
5'-UMP + H2O | high activity | Myxococcus xanthus | ? | - |
? | |
additional information | 3',5'-cGMP is either not hydrolysed or is cleaved very slowly by PdeA and PdeB, PdeA and PdeB show no activities toward 2'-AMP and 2'-GMP | Myxococcus xanthus | ? | - |
? | |
additional information | PdeA and PdeB hydrolyze 3',5'- and 2',3'-cyclic AMP to adenosine, and also demonstrate phosphatase activity toward nucleoside 5'-tri-, 5'-di-, 5'- and 3'-monophosphates with highest activities for nucleoside 5'-monophosphates | Myxococcus xanthus | ? | - |
? | |
UDP-D-glucose + H2O | UDP-D-glucose is hydrolyzed by PdeB, but not by PdeA | Myxococcus xanthus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PdeA | has 3',5'- and 2',3'-cAMP phosphodiesterase and phosphomonoesterase activities | Myxococcus xanthus |
PdeB | has 3',5'- and 2',3'-cAMP phosphodiesterase and phosphomonoesterase activities | Myxococcus xanthus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the optimum temperature for the 3',5'-cAMP phosphodiesterase activity of PdeA is 40°C | Myxococcus xanthus |
50 | - |
the optimum temperature for the 3',5'-cAMP phosphodiesterase activity of PdeA is 50°C | Myxococcus xanthus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | - |
Myxococcus xanthus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
2',3'-cAMP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.00093 | - |
3',5'-cAMP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.00096 | - |
2',3'-cAMP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.00096 | - |
3',5'-cAMP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.00155 | - |
5'-ATP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.0031 | - |
5'-AMP | PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.00454 | - |
5'-ATP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus | |
0.00551 | - |
5'-AMP | PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C | Myxococcus xanthus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | for 3',5'-cAMP with 0.1 M Tris-HCl buffer | Myxococcus xanthus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | PdeB shows no 3',5'-cAMP phosphodiesterase activity at or below pH 6 | Myxococcus xanthus |