Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in Mycobacterium leprae | Mycobacterium tuberculosis |
overexpression in Mycobacterium smegmatis and Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
homology modeling of enzyme, based on kidny bean PAP and Mre nuclease | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D21A | specific activity for cAMP hydrolysis is nearly identical to wild-type value | Mycobacterium tuberculosis |
D21A | 90% loss of catalytic activity | Mycobacterium tuberculosis |
D66A | specific activity for cAMP hydrolysis is 4fold lower than wild-type value | Mycobacterium tuberculosis |
D66A | 75% of wild-type activity, sigmoidal kinetics with respect to Mn2+ | Mycobacterium tuberculosis |
H169A | 60% of wild-type activity | Mycobacterium tuberculosis |
H169A | specific activity for cAMP hydrolysis is nearly identical to wild-type value | Mycobacterium tuberculosis |
H207A | 30% of wild-type activity | Mycobacterium tuberculosis |
H207A | specific activity for cAMP hydrolysis is 2.4 fold lower than wild-type value | Mycobacterium tuberculosis |
H23A | 60% of wild-type activity | Mycobacterium tuberculosis |
H23A | specific activity for cAMP hydrolysis is 1.4fold higher than wild-type value | Mycobacterium tuberculosis |
additional information | overexpression in Escherichia coli and in Mycobacterium leprae results in reduced intracellular cAMP levels | Mycobacterium tuberculosis |
N97A | almost complete loss of activity | Mycobacterium tuberculosis |
N97A | specific activity for cAMP hydrolysis is nearly identical to wild-type value | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete loss of activity for mutants H23A, H169A, H207A | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
3',5'-cAMP | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Km value and Hill constant for wild-type 0.021 mM and 1, respectively, for mutant D21A 0.021 mM and 4.1, respectively, for mutant H23A 0.053 mM and 1, respectively, for mutant D66A, 0.072 and 6.4, respectively, for mutant H169A 0.022 and 1.7, respectively, for mutant H207A, 0.062 and 3.1, respectively | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | Rv0805 protein could have an important role to play in regulating cAMP levels in Mycobacterium tuberculosis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis | - |
product of Rv0805 gene | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cAMP + H2O | - |
Mycobacterium tuberculosis | 5'-AMP | - |
? | |
3',5'-cGMP + H2O | - |
Mycobacterium tuberculosis | 5'-GMP | - |
? | |
additional information | Rv0805 protein could have an important role to play in regulating cAMP levels in Mycobacterium tuberculosis | Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rv0805 protein | - |
Mycobacterium tuberculosis |