Any feedback?
Literature summary for 3.1.4.16 extracted from
- Chloride ion as a modifier of 2,3-cyclic phosphodiesterase purified from halophilic Vibrio alginolyticus (1969), Biochim. Biophys. Acta, 171, 89-102.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Br- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus |  |
| CH3COO- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
| Cl- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
| HCOO- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
| I- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
| additional information | monovalent cations activate in the order of decreasing effectiveness: Cl-, Br-/I-, NO3-, HCOO-, CH3COO- | Vibrio alginolyticus | |
| NO3- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 0.2 mM, 30% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 20% inhibition of 3'-AMP hydrolysis | Vibrio alginolyticus | |
| Hg2+ | 0.2 mM, 35% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 36% inhibition of 3'-AMP hydrolysis | Vibrio alginolyticus | |
| MgCl2 | maximal inhibition at 20 mM | Vibrio alginolyticus | |
| MgSO4 | slight inhibition | Vibrio alginolyticus | |
| Na2SO4 | slight inhibition | Vibrio alginolyticus | |
| Zn2+ | 0.2 mM, 90% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 78% inhibition of 3'-AMP hydrolysis | Vibrio alginolyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
3'-CMP | - |
Vibrio alginolyticus | |
| 0.05 | - |
3'-UMP | - |
Vibrio alginolyticus | |
| 0.08 | - |
3'-AMP | - |
Vibrio alginolyticus | |
| 0.1 | - |
3'-GMP | - |
Vibrio alginolyticus | |
| 0.1 | - |
cyclic 2',3'-CMP | - |
Vibrio alginolyticus | |
| 0.2 | - |
di-p-nitrophenyl phosphate | with 200 mM NaCl | Vibrio alginolyticus | |
| 0.36 | - |
cyclic 2',3'-UMP | - |
Vibrio alginolyticus | |
| 0.45 | - |
di-p-nitrophenyl phosphate | without NaCl | Vibrio alginolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | results might indicate a surface localization | Vibrio alginolyticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.2 mM, 6-10% stimulation | Vibrio alginolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Vibrio alginolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio alginolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio alginolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Vibrio alginolyticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 8.0, 20 mM Tris buffer, stable for at least 6 months | Vibrio alginolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-AMP + H2O | - |
Vibrio alginolyticus | adenosine + phosphate | - |
? | |
| 3'-CMP + H2O | - |
Vibrio alginolyticus | cytidine + phosphate | - |
? | |
| 3'-GMP + H2O | - |
Vibrio alginolyticus | guanosine + phosphate | - |
? | |
| 3'-UMP + H2O | - |
Vibrio alginolyticus | uridine + phosphate | - |
? | |
| cyclic 2',3'-CMP + H2O | - |
Vibrio alginolyticus | 3'-CMP | - |
? | |
| cyclic 2',3'-GMP + H2O | - |
Vibrio alginolyticus | 3'-GMP | - |
? | |
| cyclic 2',3'-UMP + H2O | - |
Vibrio alginolyticus | 3'-UMP | - |
? | |
| di-p-nitrophenyl phosphate + H2O | - |
Vibrio alginolyticus | p-nitrophenol + p-nitrophenyl phosphate | - |
? | |
| additional information | - |
Vibrio alginolyticus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, in absence of salts, 55% loss of activity, in presence of 0.2 mM MnCl2 13% loss of activity | Vibrio alginolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
hydrolysis of 3'-UMP | Vibrio alginolyticus |
| 7.8 | - |
hydrolysis of 3'-CMP | Vibrio alginolyticus |
| 8.1 | - |
hydrolysis of 3'-GMP | Vibrio alginolyticus |
| 8.3 | - |
hydrolysis of 3'-AMP or di-p-nitrophenyl phosphate | Vibrio alginolyticus |
| 8.4 | - |
cyclic phosphodiesterase activity with cyclic 2',3'-phosphate | Vibrio alginolyticus |
| 8.6 | - |
cyclic phosphodiesterase activity with cyclic 2',3'-phosphate | Vibrio alginolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9 | pH 7.2: about 45% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of 3'-GMP | Vibrio alginolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
37°C, loss of activity below | Vibrio alginolyticus |
| 6 | - |
stable for at least 2 h at 37°C | Vibrio alginolyticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
