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Literature summary for 3.1.4.14 extracted from
- Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli (1990), J. Bacteriol., 172, 5445-5449.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CHAPS | - |
Escherichia coli |  |
| Triton X-100 | biphasic, optimal stimulation at 1 mM | Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| 90°C, 1% SDS, 10 min, 20% loss of activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SDS | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | increased activity in combination with Mg2+ | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-carrier protein + H2O | Escherichia coli | - |
4'-phosphopantetheine + apoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-carrier protein + H2O | - |
Escherichia coli | 4'-phosphopantetheine + apoprotein | - |
? | |
| E. coli acyl-carrier protein + H2O | - |
Escherichia coli | 4'-phosphopantetheine + apoprotein of acyl-carrier protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACP phosphodiesterase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
loss of activity after 15 min in the absence of SDS | Escherichia coli |
| 90 | - |
stable in the presence of 1% SDS | Escherichia coli |
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