Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine-specific phospholipase C | increases the N-SMase activity up to 2fold | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D609 | 48% inhibition of the enzyme in chromain after 30 min of treatment, no inhibition by D609 in the nuclear membrane | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromatin | - |
Rattus norvegicus | 785 | - |
nuclear membrane | - |
Rattus norvegicus | 31965 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | a phosphatidylcholine/sphingomyelin metabolism crosstalk which regulates the intranuclear ceramide/diacylglycerol pool exists in the chromatin structure, effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, overview | ? | - |
? | |
sphingomyelin + H2O | Rattus norvegicus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000025 | - |
chromatin | Rattus norvegicus |
0.0009 | - |
nuclear membrane | Rattus norvegicus |
0.052 | - |
phospholipid pre-incubated nuclear membrane | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a phosphatidylcholine/sphingomyelin metabolism crosstalk which regulates the intranuclear ceramide/diacylglycerol pool exists in the chromatin structure, effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, overview | Rattus norvegicus | ? | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-SMase | - |
Rattus norvegicus |
neutral sphingomyelinase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at, nuclear membranes | Rattus norvegicus |
8.4 | - |
assay at, chromatin | Rattus norvegicus |