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Literature summary for 3.1.4.12 extracted from
- His151 and His296 are the acid-base catalytic residues of Bacillus cereus sphingomyelinase in sphingomyelin hydrolysis (2003), Biol. Pharm. Bull., 26, 920-926.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of nSMase in Bacillus brevis 47 | Bacillus cereus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H151A | 0.6% of wild-type nSMase activity | Bacillus cereus |
| H151D | no nSMase activity | Bacillus cereus |
| H151E | no nSMase activity | Bacillus cereus |
| H151F | no nSMase activity | Bacillus cereus |
| H151K | no nSMase activity | Bacillus cereus |
| H151N | no nSMase activity | Bacillus cereus |
| H151Q | 8.3% of wild-type nSMase activity | Bacillus cereus |
| H151R | no nSMase activity | Bacillus cereus |
| H151Y | no nSMase activity | Bacillus cereus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
sphingomyelin | 37°C, pH 7.5 | Bacillus cereus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | extracellular soluble toxin that induces hemolysis by acting on sphingomyelin-rich erythrocytes of mammals such as ruminants | Bacillus cereus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can replace Mg2+ | Bacillus cereus | |
| Mg2+ | required for activity, Glu53 act as a ligand for the essential Mg2+ | Bacillus cereus | |
| Mn2+ | can replace Mg2+ | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | Bacillus cereus | - |
N-acylsphingosine + choline phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant nSMase | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hexadecanoylamino-4-nitrophenylphosphocholine + H2O | - |
Bacillus cereus | 2-hexadecanoylamino-4-nitrophenol + choline phosphate | - |
? | |
| sphingomyelin + H2O | - |
Bacillus cereus | N-acylsphingosine + choline phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral sphingomyelinase | - |
Bacillus cereus |
| nSMase | - |
Bacillus cereus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.6 | - |
sphingomyelin | 37°C, pH 7.5 | Bacillus cereus | |
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