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Literature summary for 3.1.4.12 extracted from
- Expression of recombinant human acid sphingomyelinase in insect Sf21 cells: purification, processing and enzymatic characterization (1998), J. Biotechnol., 63, 29-40.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in insect, Spodoptera frugiperda 21cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
sphingomyelin | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
infection of insect Sf21cells with recombinant baculovirus | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 370fold | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
different assay conditions, substrates etc. | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6.5 | - |
Homo sapiens |
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