Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Detergents | - |
Bacillus cereus | |
sodium deoxycholate | - |
Bacillus cereus | |
Triton X-100 | activation with sphingomyelin | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | when sphingomyelin as substrate with Triton X-100 is used, competitive inhibitor against Mg2+ | Bacillus cereus | |
cetyltrimethylammonium bromide | - |
Bacillus cereus | |
EDTA | - |
Bacillus cereus | |
Triton X-100 | slight inhibition with 2-hexadecynoylamino-4-nitrophenylphosphorylcholine | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine | in presence of Mg2+ | Bacillus cereus | |
0.038 | - |
sphingomyelin | in presence of Mg2+ and Triton X-100 | Bacillus cereus | |
0.17 | - |
sphingomyelin | in presence of Mg2+ and sodium deoxycholate | Bacillus cereus | |
0.174 | - |
hexadecanoyl-p-nitrophenyl phosphorylcholine | - |
Bacillus cereus | |
0.405 | - |
sphingomyelin | in presence of Triton X-100 | Bacillus cereus | |
0.565 | - |
sphingomyelin | in presence of sodium deoxycholate | Bacillus cereus | |
11.6 | - |
4-nitrophenyl phosphorylcholine | - |
Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation when water-soluble substrates are used | Bacillus cereus | |
Mg2+ | - |
Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23300 | - |
sedimentation equilibrium centrifugation | Bacillus cereus |
41000 | - |
x * 41000, SDS-PAGE | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Bacillus cereus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2200fold | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O | - |
Bacillus cereus | 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate | - |
? | |
4-nitrophenyl phosphorylcholine + H2O | - |
Bacillus cereus | 4-nitrophenol + phosphorylcholine | - |
? | |
hexadecanoyl-p-nitrophenylphosphorylcholine + H2O | - |
Bacillus cereus | hexadecanoyl-p-nitrophenol + choline phosphate | - |
? | |
lysophosphatidylcholine + H2O | - |
Bacillus cereus | 1-acylglycerol + choline phosphate | - |
? | |
p-nitrophenylphosphorylcholine + H2O | - |
Bacillus cereus | choline phosphate + p-nitrophenol | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE | Bacillus cereus |