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Literature summary for 3.1.4.12 extracted from
- Release of sphingomyelin phosphodiesterase (acid sphingomyelinase) by ammonium chloride from CL 1D mouse L-cells and human fibroblasts. Partial purification and characterization of the exported enzymes (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 863-871.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Mus musculus |  |
| Triton X-100 | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
sphingomyelin | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | in presence of NH4Cl | Mus musculus | - |
- |
| extracellular | in presence of NH4Cl | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration or gel electrophoresis | Mus musculus |
| 68000 | - |
gel filtration or gel electrophoresis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 134fold | Homo sapiens |
| 64fold | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | - |
Homo sapiens | - |
| L-cell | cell line | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
different assay conditions, substrates etc. | Mus musculus |
| additional information | - |
different assay conditions, substrates etc. | Homo sapiens |
| additional information | - |
normal and Niemann Pick disease type C fibroblasts | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several weeks | Mus musculus |
| 4°C, stable for several weeks | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
20 min, 40% activity | Mus musculus |
| 56 | - |
30 min, 40% activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Homo sapiens |
| 4.8 | - |
- |
Mus musculus |
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