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Literature summary for 3.1.4.11 extracted from

  • Li, L.; Wang, F.; Yan, P.; Jing, W.; Zhang, C.; Kudla, J.; Zhang, W.
    A phosphoinositide-specific phospholipase C pathway elicits stress-induced Ca2+ signals and confers salt tolerance to rice (2017), New Phytol., 214, 1172-1187 .
    View publication on PubMed
Search for more literature for 3.1.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed as glutathione S-transferase-tagged protein in Escherichia coli Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
D161R the mutant shows 10% and 5% hydrolyzing activity for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and 1-phosphatidyl-1D-myo-inositol 4-phosphate, respectively, compared to the wild type enzyme Oryza sativa Japonica Group
H129A the mutant shows less than 22% and 6% hydrolyzing activity for 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and 1-phosphatidyl-1D-myo-inositol 4-phosphate, respectively, compared to the wild type enzyme Oryza sativa Japonica Group

Inhibitors

Inhibitors Comment Organism Structure
EGTA
-
 Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryza sativa Japonica Group 5829
-
plasma membrane
-
 Oryza sativa Japonica Group 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximum activity at 0.1 mM Ca2+ Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Oryza sativa Japonica Group
-
 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O Oryza sativa Japonica Group preferred substrate. With 1-phosphatidyl-1D-myo-inositol 4-phosphate, the enzyme displays a more than 3fold higher hydrolyzing activity compared to 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1D-myo-inositol 1,4-bisphosphate + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group
-
 cultivar Nipponbare
-

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Oryza sativa Japonica Group 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O preferred substrate Oryza sativa Japonica Group 1D-myo-inositol 1,4-bisphosphate + diacylglycerol
-
 ?
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O preferred substrate. With 1-phosphatidyl-1D-myo-inositol 4-phosphate, the enzyme displays a more than 3fold higher hydrolyzing activity compared to 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Oryza sativa Japonica Group 1D-myo-inositol 1,4-bisphosphate + diacylglycerol
-
 ?
additional information the enzyme displays scarcely any activity towards 1-phosphatidyl-1D-myo-inositol or phosphatidylcholine Oryza sativa Japonica Group ?
-
 ?

Synonyms

Synonyms Comment Organism
phosphoinositide-specific phospholipase C
-
 Oryza sativa Japonica Group
PI-PLC
-
 Oryza sativa Japonica Group
PLC1
-
 Oryza sativa Japonica Group

General Information

General Information Comment Organism
malfunction loss of enzyme function renders rice plants salt sensitive Oryza sativa Japonica Group
physiological function the enzyme elicits stress-induced Ca2+ signals regulating salt tolerance in rice. Enzyme-mediated Ca2+ signaling is essential for controlling Na+ accumulation in leaf blades, thus establishing whole plant salt tolerance Oryza sativa Japonica Group