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Literature summary for 3.1.4.11 extracted from

  • Cerminati, S.; Paoletti, L.; Peiru, S.; Menzella, H.G.; Castelli, M.E.
    The betagamma-crystallin domain of Lysinibacillus sphaericus phosphatidylinositol phospholipase C plays a central role in protein stability (2018), Appl. Microbiol. Biotechnol., 102, 6997-7005 .
    View publication on PubMed
Search for more literature for 3.1.4.11

Application

Application Comment Organism
food industry the enzyme is used in industrial soybean oil degumming Lysinibacillus sphaericus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21AI cells Lysinibacillus sphaericus

General Stability

General Stability Organism
the presence of Ca2+ increases the thermal and chemical stability of the protein both in aqueous media and in oil Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the presence of Ca2+ increases the thermal and chemical stability of the protein both in aqueous media and in oil Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Lysinibacillus sphaericus
-
 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Lysinibacillus sphaericus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
butyl-fluorescein myo-inositol phosphate + H2O
-
 Lysinibacillus sphaericus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 451600, His-tagged enzyme, calculated from amino acid sequence Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
phosphatidylinositol phospholipase C
-
 Lysinibacillus sphaericus
PIPLC
-
 Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 without Ca2+, the full-length protein retains 85% of its initial activity after treatment at 50°C. The enzyme is almost completely inactivated after incubation at 60°C .The enzyme retains more than 99% of its initial activity at 50°C and over 40% at 60°C in the presence of Ca2+. Lysinibacillus sphaericus