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Literature summary for 3.1.4.11 extracted from

  • Moroz, O.V.; Blagova, E.; Lebedev, A.A.; Norgaard, A.; Segura, D.R.; Blicher, T.H.; Brask, J.; Wilson, K.S.
    The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium (2017), Acta crystallogr. Sect. D, 73, 32-44 .
    View publication on PubMed
Search for more literature for 3.1.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Pseudomonas sp. 62186

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate, 24-27% (w/v) PEG 3350, 0.1 M bis-triazinylpyridine pH 10.0 Pseudomonas sp. 62186

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Pseudomonas sp. 62186

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Pseudomonas sp. 62186
-
 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. 62186 A0A1S4NYD4
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 gel filtration and Source 15S column chromatography Pseudomonas sp. 62186

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Pseudomonas sp. 62186 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
soybean oil + H2O
-
 Pseudomonas sp. 62186 ?
-
 ?

Synonyms

Synonyms Comment Organism
phosphoinositide-specific phospholipase C
-
 Pseudomonas sp. 62186
PI-PLC
-
 Pseudomonas sp. 62186