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Literature summary for 3.1.4.11 extracted from
- Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs (2004), J. Biol. Chem., 279, 10408-10412.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| microinjection of recombinant PLCzeta protein into mouse eggs induces serial Ca2+ spikes quite similar to that produced by the injection of sperm extract, probably because of repetitive Ca2+ release from the endoplasmic reticulum caused by continuously produced inositol 1,4,5-trisphosphate | Mus musculus |
| PLC-delta1 | Mus musculus |
| PLCdelta1, expression in Sf9 cells | Mus musculus |
| PLCzeta, expression in Sf9 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | PLCzeta mutant lacking three EF-hand domains | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates PLCdelta1, EC50: 0.0057 mM, PLCdelta1 has a 100fold lower Ca2+ sensitivity than PLCzeta | Mus musculus |  |
| Ca2+ | PLCzeta, 70% of maximal activity at 100 nM Ca2+, EC50: 52 nM, PLCzeta has a 100fold higher Ca2+ sensitivity than PLCdelta1 | Mus musculus | |
| Ca2+ | activity of PLC-delta1 increases at a Ca2+ concentration between 0.001 and 0.03 mM | Mus musculus | |
| Ca2+ | PLCzeta exhibites a significant activity at Ca2+ concentration of 10 nM and has 70% maximal activity at 100 nM Ca2+ that is the basal intracellular calcium ion concentration level of cells | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
x * 78000, recombinant His-tagged PLCzeta, SDS-PAGE | Mus musculus |
| 90000 | - |
x * 90000, recombinant His-tagged PLCdelta1, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | Mus musculus | sperm-specific PLCzeta induces intracellular Ca2+ oscillations and subsequent early embryonic development when expressed in mouse eggs, PLCzeta may be the physiological mammalian egg-activating sperm factor | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| additional information | Mus musculus | recombinant PLCzeta protein induces Ca2+ oscillations in mouse eggs | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Mus musculus | Q8K4D7 | - |
- |
| Mus musculus | Q8K4D7 | PLCzeta | - |
| Mus musculus | Q8R3B1 | PLCdelta1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme PLC-delta1 prepared by a baculovirus expression system | Mus musculus |
| enzyme prepared by a baculovirus expression system | Mus musculus |
| recombinant PLCdelta1 | Mus musculus |
| recombinant PLCzeta | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Mus musculus | - |
| sperm | sperm-specific PLCzeta | Mus musculus | - |
| testis | - |
Mus musculus | - |
| testis | PLCzeta | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
PLCzeta, at 0.001 mM Ca2+, pH 7, 37°C | Mus musculus |
| 1.3 | - |
at 0.001 mM Ca2+ | Mus musculus |
| 65 | - |
PLCdelta1, at 0.03 mM Ca2+, pH 7, 37°C | Mus musculus |
| 65 | - |
at 0.03 mM Ca2+, PLC-delta1 | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Mus musculus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | domain structure of PLCdelta1 | Mus musculus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | domain structure of PLCzeta | Mus musculus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | sperm-specific PLCzeta induces intracellular Ca2+ oscillations and subsequent early embryonic development when expressed in mouse eggs, PLCzeta may be the physiological mammalian egg-activating sperm factor | Mus musculus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| additional information | recombinant PLCzeta protein induces Ca2+ oscillations in mouse eggs | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, recombinant His-tagged PLCzeta, SDS-PAGE | Mus musculus |
| ? | x * 90000, recombinant His-tagged PLCdelta1, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PLC-delta1 | - |
Mus musculus |
| PLC-zeta | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Mus musculus |
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