Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | concentrations above 1 mM are inhibitory | Homo sapiens | |
EDTA | inhibition by EDTA can be reverted upon the addition of increasing concentrations of Ca2+ and Mg2+ in the assay | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm | Homo sapiens | 5829 | - |
membrane | PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | high salt concentration, (NH4)2SO4, 2 M, dissociates the high molecular weight form yielding the low molecular form and increasing the specific activity | Homo sapiens | |
Ca2+ | PIPLC (cytosol) presents a total dependence on the presence of Ca2+ in the assay, which cannot be substituted by other divalent cations (Mg2+, Mn2+, Zn2+) | Homo sapiens | |
Mg2+ | no requirement for the membrane enzyme solubilized with sodium cholate, activates the membrane enzyme after solubilization in octyl glucoside with maximal activity at 0.1 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
PI-PLC, can be resolved into two peaks of activity of high M (60000-70000) and low M (16000-18000) | Homo sapiens |
18000 | - |
purified Pi-PLC (membrane) has a molecular weight of about 18000, SDS-PAGE or gel filtration | Homo sapiens |
57000 | - |
purified Pi-PLC (cytosol), SDS-PAGE or gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-acyl-2-arachidonoylphosphatidylinositol + H2O | - |
Homo sapiens | ? | - |
? | |
1-acyl-2-linoleoylphosphatidylinositol + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | no phospholipase A2 activity is found in the purified enzymes | Homo sapiens | ? | - |
? | |
phosphatidylinositol + H2O | Pi-PLC (membrane) and Pi-PLC (cytosol) are specific for this substrate | Homo sapiens | diacylglycerol + 1D-myo-inositol 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
low molecular weight PI-PLC | - |
Homo sapiens |
phosphatidylinositol-specific phospholipases C | - |
Homo sapiens |
PI-PLC | - |
Homo sapiens |
PI-PLCc | - |
Homo sapiens |
PI-PLCm | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Pi-PLC (cytosol) | Homo sapiens |
7 | - |
Pi-PLC (membrane) solubilized with sodium cholate | Homo sapiens |
7.8 | - |
assay at | Homo sapiens |
8 | - |
Pi-PLC (membrane) solubilized with octyl glucoside | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
both PI-PLC (membrane) and PI-PLC (cytosol) are fairly stable proteins when incubated for short periods of time (30 min) to a wide range of pH. PI-PLC (membrane) shows a fall of about 20-30% activity below pH 5.0 and is stable to alkaline pH up to 11 | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | purified Pi-PLC (cytosol) | - |
6.2 |
Homo sapiens | purified Pi-PLC (membrane) | 9.2 | 9 |