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Literature summary for 3.1.4.11 extracted from

  • Allan, D.; Michell, R.H.
    Phosphatidylinositol cleavage catalysed by the soluble fraction from lymphocytes. Activity at pH 5.5 and pH 7.0 (1974), Biochem. J., 142, 591-597.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
chlorpromazine stimulates at pH 5.5 Sus scrofa
Cinchocaine stimulates at pH 5.5 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 above 0.4 mM, pH 7.0 Sus scrofa
chlorpromazine at pH 7.0 Sus scrofa
Cinchocaine at pH 7.0 Sus scrofa
MgCl2 above 0.4 mM, pH 7.0 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 stimulates at pH 5.5, pH dependent Sus scrofa
K+ stimulates at pH 5.5 Sus scrofa
MgCl2 stimulates at pH 5.5, but less active than CaCl2, pH dependent Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte from mesenteric lymph nodes Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity at pH 7.0 (and 0.4 mM CaCl2) is decreased by agents, such as salts and lipophilic cations, which tend to neutralize the negative charge of phosphatidylinositol. At pH 5.5 these agents slightly stimulate activity Sus scrofa
0.025
-
 pH 7.3, pellet Sus scrofa
0.034
-
 pH 5.4, pellet Sus scrofa
0.082
-
 pH 7.3, cell pellet Sus scrofa
0.088
-
 pH 5.4, cell pellet Sus scrofa
0.26
-
 pH 7.3, supernatant (soluble) Sus scrofa
0.28
-
 pH 5.4, supernatant (soluble) Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, stable for several month Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity is detectable towards either phosphatidylcholine or phosphatidylethanolamine Sus scrofa ?
-
 ?
phosphatidylinositol + H2O
-
 Sus scrofa 1,2-diacylglycerol + 1D-myo-inositol 1-phosphate 1D-myo-inositol 1,2-cyclic phosphate is also formed ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 5.6 5.4-5.6 and 7.0-7.3 Sus scrofa
5.6
-
 assay at Sus scrofa
7 7.3 5.4-5.6 and 7.0-7.3 Sus scrofa
7.1
-
 assay at Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5
-
 Sus scrofa