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Literature summary for 3.1.4.11 extracted from
- Characterization of phosphatidylinositol-specific phospholipase C from cultured vascular smooth muscle cells (1991), J. Biol. Chem., 266, 15498-15504.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | inhibits phosphatidylinositol hydrolysis of low molecular weight PLC. Conversely, PIP2 hydrolysis is stimulated by increasing concentrations of NaCl | Rattus norvegicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | PLC requires calcium for full activation | Rattus norvegicus | |
| NaCl | inhibits phosphatidylinositol hydrolysis of low molecular weight PLC. Conversely, PIP2 hydrolysis is stimulated by increasing concentrations of NaCl | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE, low molecular weight PLC hydrolyzes both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate has a molecular mass of 58 kDa. Cloning techniques have tentatively identified the low molecular weight peak as PLC-alpha | Rattus norvegicus |
| 66000 | - |
low molecular PLC by size exclusion chromatography | Rattus norvegicus |
| 145000 | - |
high molecular PLC shows much greater activity against phosphatidylinositol than phosphatidylinositol 4,5-bisphosphate and is active at lower calcium concentrations | Rattus norvegicus |
| 157000 | - |
high molecular PLC by size exclusion chromatography | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | PLC activity in vascular smooth muscle cells can be divided into two types based on their calcium and pH requirements, substrate preferences, and molecular weights | ? | - |
? | |
| additional information | Rattus norvegicus | several different assay conditions for both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate are tested on samples of crude supernatant for their ability to support PLC activity | ? | - |
? | |
| additional information | Rattus norvegicus | the injection fraction shows no activity against phosphatidylcholine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purified, DEAE-cellulose and heparin-Sepharose chromatographies followed by Mono Q and size exclusion HPLC | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | cultured vascular smooth muscle cell, isolated from rat thoracic aorta | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
PLC activity in vascular smooth muscle cells can be divided into two type based on their calcium and pH requirements, substrate preferences, and molecular weights | Rattus norvegicus |
| 28.97 | - |
phosphatidylinositol as substrate, last purification step | Rattus norvegicus |
| 204.1 | - |
phosphatidylinositol 4,5-bisphosphate as substrate, last purification step | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| Partially purified PLC can be stored in buffer C at -80°C for several months without appreciable loss of activity. However, repeated freezing and thawing has a deleterious effect on enzyme activity. More purified PLC (Mono Q and size exclusion fractions) can be stored at 4°C for 5-7 days with a gradual loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PLC activity in vascular smooth muscle cells can be divided into two types based on their calcium and pH requirements, substrate preferences, and molecular weights | Rattus norvegicus | ? | - |
? | |
| additional information | several different assay conditions for both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate are tested on samples of crude supernatant for their ability to support PLC activity | Rattus norvegicus | ? | - |
? | |
| additional information | the injection fraction shows no activity against phosphatidylcholine | Rattus norvegicus | ? | - |
? | |
| phosphatidylinositol + H2O | low molecular weight PLC hydrolyzes both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate, high molecular PLC shows much greater activity against phosphatidylinositol than phosphatidylinositol 4,5-bisphosphate | Rattus norvegicus | ? | - |
? | |
| phosphatidylinositol 4,5-bisphosphate + H2O | enzyme activities for phosphatidylinositol 4,5-bisphosphate are strongly dependent on the relative levels of calcium and pH in the assay buffer | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphatidylinositol-specific phospholipase C | - |
Rattus norvegicus |
| PLC | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
high molecular PLC (157 kDa), phosphatidylinositol as substrate | Rattus norvegicus |
| 6 | - |
at the higher calcium concentration the pH optimum for phosphatidylinositol 4,5-bisphosphate hydrolysis is 6.0 | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Rattus norvegicus |
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