Activating Compound | Comment | Organism | Structure |
---|---|---|---|
albumin | maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml | Rattus norvegicus | |
cytochrome c | maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml | Rattus norvegicus | |
histone | maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml | Rattus norvegicus | |
additional information | rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1. Incubation with the kinase increases the activity of both enzymes however, possibly by phosphorylation of another protein in the preparations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
phosphatidylinositol | isoenzyme PLC-gamma | Rattus norvegicus | |
0.031 | - |
phosphatidylinositol | isoenzyme PLC-gamma | Rattus norvegicus | |
0.035 | - |
phosphatidylinositol | isoenzyme PLC-beta1 | Rattus norvegicus | |
0.058 | - |
phosphatidylinositol 4,5-bisphosphate | isoenzyme PLC-beta1 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolute requirement for both isoenzymes. Maximal activity with phosphatidylinositol at pH 5.5: 1 mM. Maximal activity with phosphatidylinositol 4,5-diphosphate at pH 7.0: 0.1 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1 | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
isoenzyme PLC-gamma | Rattus norvegicus |
11.4 | - |
isoenzyme PLC-beta1 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol + H2O | phosphatidylinositol 4,5-bisphosphate is a better substrate | Rattus norvegicus | ? | - |
? | |
phosphatidylinositol 4,5-bisphosphate + H2O | - |
Rattus norvegicus | D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | isoenzyme PLC beta1 produces 21% inositol 1:2,4,5 cyclic trisphosphate at pH 5.5 and 7.5% at pH 7.0 | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | hydrolysis of phosphatidylinositol, isoenzyme PLC-beta-1 and PLC-gamma, in presence of 1 mM CaCl2 | Rattus norvegicus |
6.5 | 7 | hydrolysis of phosphatidylinositol, isoenzyme PLC-beta1 in presence of 0.6 mg/ml sodium deoxycholate, isoenzyme PLC-gamma in presence of 0.4 mg/ml sodium deoxycholate | Rattus norvegicus |
7.5 | - |
hydrolysis of phosphatidylinositol 4,5-diphosphate, in presence of 0.1 mM CaCl2 | Rattus norvegicus |