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Literature summary for 3.1.4.11 extracted from

  • Blank, J.L.; Foster, K.A.; Hawthorne, J.N.
    Purification, properties, and phosphorylation by protein kinase C of two phosphoinositidase C isoenzymes from rat brain (1991), J. Neurochem., 57, 15-21.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
albumin maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml Rattus norvegicus
cytochrome c maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml Rattus norvegicus
histone maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml Rattus norvegicus
additional information rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1. Incubation with the kinase increases the activity of both enzymes however, possibly by phosphorylation of another protein in the preparations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
phosphatidylinositol isoenzyme PLC-gamma Rattus norvegicus
0.031
-
phosphatidylinositol isoenzyme PLC-gamma Rattus norvegicus
0.035
-
phosphatidylinositol isoenzyme PLC-beta1 Rattus norvegicus
0.058
-
phosphatidylinositol 4,5-bisphosphate isoenzyme PLC-beta1 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement for both isoenzymes. Maximal activity with phosphatidylinositol at pH 5.5: 1 mM. Maximal activity with phosphatidylinositol 4,5-diphosphate at pH 7.0: 0.1 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
isoenzyme PLC-gamma Rattus norvegicus
11.4
-
isoenzyme PLC-beta1 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol + H2O phosphatidylinositol 4,5-bisphosphate is a better substrate Rattus norvegicus ?
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
-
Rattus norvegicus D-myo-inositol 1,4,5-trisphosphate + diacylglycerol isoenzyme PLC beta1 produces 21% inositol 1:2,4,5 cyclic trisphosphate at pH 5.5 and 7.5% at pH 7.0 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 hydrolysis of phosphatidylinositol, isoenzyme PLC-beta-1 and PLC-gamma, in presence of 1 mM CaCl2 Rattus norvegicus
6.5 7 hydrolysis of phosphatidylinositol, isoenzyme PLC-beta1 in presence of 0.6 mg/ml sodium deoxycholate, isoenzyme PLC-gamma in presence of 0.4 mg/ml sodium deoxycholate Rattus norvegicus
7.5
-
hydrolysis of phosphatidylinositol 4,5-diphosphate, in presence of 0.1 mM CaCl2 Rattus norvegicus