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Literature summary for 3.1.4.11 extracted from

  • Wang, P.; Toyoshima, S.; Osawa, T.
    Partial purification and characterization of membrane-bound and cytosolic phosphatidylinositol-specific phospholipases C from murine thymocytes (1986), J. Biochem., 100, 1015-1022.
    View publication on PubMed
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
arachidonic acid enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
diacylglycerol enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
phosphatidic acid enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
phosphatidylcholine enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme Mus musculus
phosphatidylethanolamine enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
phosphatidylserine enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
membrane bound to Mus musculus 16020
-
microsome
-
 Mus musculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000 70000 membrane-bound enzyme, in presence of sodium cholate, gel filtration Mus musculus
71000 72000 cytosolic enzyme, in presence of sodium deoxycholate, gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
thymocyte
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.114
-
 membrane-bound enzyme Mus musculus
0.235
-
 cytosolic enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol + H2O at neutral pH and low Ca2+ concentration the membrane bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol Mus musculus ?
-
 ?
phosphatidylinositol 4,5-bisphosphate + H2O membrane-bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol Mus musculus D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 hydrolysis of phosphatidylinositol, membrane bound enzyme and cytosolic enzyme Mus musculus
7.5
-
 hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus