Protein Variants | Comment | Organism |
---|---|---|
H182A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme, potentiates the cytotoxicity of the Hisgab mutants and induces a DNA topoisomerase 1-independent lethal phenotype in combination with the H432N mutant | Saccharomyces cerevisiae |
H182A/H432N | the mutant protein reveals a dramatic (210fold) reduction in catalytic activity compared to the wild type | Saccharomyces cerevisiae |
H182A/H432R | the mutant protein reveals a dramatic (more than 50%) reduction in catalytic activity compared to the wild type | Saccharomyces cerevisiae |
H182F | catalytically inactive | Saccharomyces cerevisiae |
H432N | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
H432R | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | wild type enzyme, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
0.0026 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H432N, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
0.0034 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H182A, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
0.0048 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H432R, at pH 7.5 and 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38319 | - |
- |
Purification (Comment) | Organism |
---|---|
SP-Sepharose column chromatography and Flag M2 affinity matrix chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5'-GATCTAAAAGACTT-3')-phosphotyrosine + H2O | - |
Saccharomyces cerevisiae | (5'-GATCTAAAAGACTT-3')-phosphate + L-tyrosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TDP1 | - |
Saccharomyces cerevisiae |
tyrosyl-DNA phosphodiesterase I | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H182A, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
0.007 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H432R, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
0.012 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H432N, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
0.28 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | wild type enzyme, at pH 7.5 and 30°C | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the repair of 3'-DNA adducts, such as the 3'-phosphotyrosyl linkage of DNA topoisomerase I to DNA | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H182A, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
1.5 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H432R, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
4.7 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | mutant enzyme H432N, at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
250 | - |
(5'-GATCTAAAAGACTT-3')-phosphotyrosine | wild type enzyme, at pH 7.5 and 30°C | Saccharomyces cerevisiae |