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Literature summary for 3.1.4.1 extracted from

  • Lebedeva, N.A.; Rechkunova, N.I.; Ishchenko, A.A.; Saparbaev, M.; Lavrik, O.I.
    The mechanism of human tyrosyl-DNA phosphodiesterase 1 in the cleavage of AP site and its synthetic analogs (2013), DNA Repair, 12, 1037-1042.
    View publication on PubMed
Search for more literature for 3.1.4.1

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme cleaves the apurinic/apyrimidinic site in DNA by hydrolysis of the phosphodiester bond between the substituent and 5' adjacent phosphate. The product of enzyme cleavage in the case of the apurinic/apyrimidinic site is unstable and is hydrolyzed with the formation of 3'- and 5'-margin phosphates. The following repair demands the ordered action of polynucleotide kinase phosphorylase, with XRCC1, DNA polymerase beta, and DNA ligase, mechanism, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity and phosphocellulose adsorption chromatography, to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-hydroxydecyl-oligonucleotide + H2O different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site Homo sapiens ?
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 ?
3-hydroxy-2(hydroxymethyl)-tetrahydrofuran-oligonucleotide + H2O different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site. In the case of tetrahydrofuran, the enzyme generates break with the 5'-tetrahydrofuran and the 3'-phosphate termini Homo sapiens ? the enzyme generates a non-phosphorylated 5'-THF-terminus by the cleavage of 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran-containing DNA ?
bis-ethylene glycolyl-oligonucleotide + H2O different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site Homo sapiens ?
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 ?
additional information the enzyme cleaves the apurinic/apyrimidinic site in DNA by hydrolysis of the phosphodiester bond between the substituent and 5' adjacent phosphate. The product of enzyme cleavage in the case of the apurinic/apyrimidinic site is unstable and is hydrolyzed with the formation of 3'- and 5'-margin phosphates. The following repair demands the ordered action of polynucleotide kinase phosphorylase, with XRCC1, DNA polymerase beta, and DNA ligase, mechanism, overview Homo sapiens ?
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 ?
additional information the enzyme is also able to effectively cleave non-nucleotide insertions in DNA, decanediol and diethyleneglycol moieties by the same mechanism as in the case of tetrahydrofuran cleavage, it catalyzes the hydrolysis of decanediol- and bis-ethylene glycol-containing DNA even more effectively than tetrahydrofuran-containing DNA particularly in the case of decanediol-containing DNA. Repair of 5'-tetrahydrofuran and other apurinic/apyrimidinic site analogues can be processed by the long-patch base excision repair pathway, overview.The efficiency of enzyme-catalyzed hydrolysis of apurinic/apyrimidinic-site analogues correlates with the DNA helix distortion induced by the substituent Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
TDP1
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 Homo sapiens
tyrosyl-DNA phosphodiesterase 1
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 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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 assay at Homo sapiens