Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Oryza sativa |
DNA and amino acid sequence determination and analysis, expression of GFP-tagged full-length isozyme NPP1 and truncated mutants in Oryza sativa cells | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme NPP1 contains a plastid-targeting signal | Oryza sativa | 9507 | - |
additional information | no targeting of isozyme NPP3 to plastids | Oryza sativa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, mature isozyme NPP1, SDS-PAGE | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryza sativa | NPP1 exhibits hydrolytic activity toward ADP-glucose and plastid-targeting ability | ? | - |
? | |
additional information | Oryza sativa | unlike isozyme NPP1, isozyme NPP3 exhibits no hydrolytic activity toward ADP-glucose and no plastid-targeting ability | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q10Q09 | isozyme NPP3; cv. Nipponbare, isozyme NPP3 | - |
Oryza sativa | Q6ZI95 | isozyme NPP1; cv. Nipponbare, isozyme NPP1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | clear difference between N-terminal proteolytic processing schemes to form mature enzyme proteins of isozymes NPP1 and NPP3 | Oryza sativa |
proteolytic modification | clear difference between N-terminal proteolytic processing schemes to form mature enzyme proteins of isozymes NPP1 and NPP3. NPP1 is matured to a 70-kDa protein by two-step proteolytic processing, proprotein processing occurs post-translationally in the ER-Golgi system, overview | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NPP1 exhibits hydrolytic activity toward ADP-glucose and plastid-targeting ability | Oryza sativa | ? | - |
? | |
additional information | unlike isozyme NPP1, isozyme NPP3 exhibits no hydrolytic activity toward ADP-glucose and no plastid-targeting ability | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, mature isozyme NPP1, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
NPP1 | - |
Oryza sativa |
NPP3 | - |
Oryza sativa |
nucleotide pyrophosphatase/phosphodiesterase 1 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Oryza sativa |