Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.1 extracted from

  • Spaeth, B.; Settele, F.; Schilling, O.; DAngelo, I.; Vogel, A.; Feldmann, I.; Meyer-Klaucke, W.; Marchfelder, A.
    Metal requirements and phosphodiesterase activity of tRNase Z enzymes (2007), Biochemistry, 46, 14742-14750.
    View publication on PubMed
Search for more literature for 3.1.4.1

Protein Variants

Protein Variants Comment Organism
C25G 3fold decrease in ratio kcat/KM value Arabidopsis thaliana
C40G 50% decrease in ratio kcat/KM value Arabidopsis thaliana
E208A 33% increase in ratio kcat/KM value Arabidopsis thaliana
F51L 50% decrease in ratio kcat/KM value Arabidopsis thaliana
G62V 80% decrease in ratio kcat/KM value Arabidopsis thaliana
P178A 65% increase in ratio kcat/KM value Arabidopsis thaliana
P64A 75% decrease in ratio kcat/KM value Arabidopsis thaliana
R252G 40fold increase in ratio kcat/KM value Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 bis(4-nitrophenyl)phosphate mutant P178A, pH 7.4, 37°C Arabidopsis thaliana
1.8
-
 bis(4-nitrophenyl)phosphate mutant R252G, pH 7.4, 37°C Arabidopsis thaliana
3.5
-
 bis(4-nitrophenyl)phosphate mutant E208A, pH 7.4, 37°C Arabidopsis thaliana
6
-
 bis(4-nitrophenyl)phosphate mutant C40G, pH 7.4, 37°C Arabidopsis thaliana
6.5
-
 bis(4-nitrophenyl)phosphate mutant F51L, pH 7.4, 37°C Arabidopsis thaliana
8
-
 bis(4-nitrophenyl)phosphate mutant G62V, pH 7.4, 37°C Arabidopsis thaliana
8.5
-
 bis(4-nitrophenyl)phosphate wild-type, pH 7.4, 37°C Arabidopsis thaliana
13.2
-
 bis(4-nitrophenyl)phosphate mutant C25G, pH 7.4, 37°C Arabidopsis thaliana
22.2
-
 bis(4-nitrophenyl)phosphate mutant P64A, pH 7.4, 37°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
additional information hydrolysis of bis(4-nitrophenyl)phosphate requires the presence of Zn2+. Pre-5RNa processing by enzyme requires additional metal ions Arabidopsis thaliana
Zn2+ high affinity for Zn2+ ions, upon incubation with metal chelators, 0.76 mol Zn2+ ions are retained per mol of enzyme dimer Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 endonuclease tRNase Z also has phosphodiesterase activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(4-nitrophenyl)phosphate + H2O
-
 Arabidopsis thaliana 4-nitrophenyl phosphate + 4-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
tRNase Z
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 bis(4-nitrophenyl)phosphate mutant G62V, pH 7.4, 37°C Arabidopsis thaliana
1.8
-
 bis(4-nitrophenyl)phosphate mutant P178A, pH 7.4, 37°C Arabidopsis thaliana
2.3
-
 bis(4-nitrophenyl)phosphate mutant F51L, pH 7.4, 37°C Arabidopsis thaliana
3.1
-
 bis(4-nitrophenyl)phosphate mutant C40G, pH 7.4, 37°C Arabidopsis thaliana
3.9
-
 bis(4-nitrophenyl)phosphate mutant C25G, pH 7.4, 37°C Arabidopsis thaliana
4
-
 bis(4-nitrophenyl)phosphate mutant E208A, pH 7.4, 37°C Arabidopsis thaliana
4.6
-
 bis(4-nitrophenyl)phosphate mutant P64A, pH 7.4, 37°C Arabidopsis thaliana
7.4
-
 bis(4-nitrophenyl)phosphate wild-type, pH 7.4, 37°C Arabidopsis thaliana
68.5
-
 bis(4-nitrophenyl)phosphate mutant R252G, pH 7.4, 37°C Arabidopsis thaliana