Application | Comment | Organism |
---|---|---|
analysis | useful tool in nucleic acid research, characterization of oligonucleotides and as an aid in sequence analysis | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Daucus carota | |
EDTA | inactivation reversed by Mg2+, Mn2+, Ca2+, Zn2+ and slightly by Co2+ | Daucus carota | |
glutathione | - |
Daucus carota | |
L-cysteine | - |
Daucus carota | |
Reducing agents | - |
Daucus carota | |
terminal 3'-monophosphoryl group of substrates | - |
Daucus carota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Daucus carota | |
Co2+ | slight activation | Daucus carota | |
divalent cations | required for activity | Daucus carota | |
Mg2+ | - |
Daucus carota | |
Mn2+ | - |
Daucus carota | |
Zn2+ | - |
Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | 115000 | gel filtration | Daucus carota |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
carrot | - |
Purification (Comment) | Organism |
---|---|
- |
Daucus carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Daucus carota | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
253.3 | - |
- |
Daucus carota |
Storage Stability | Organism |
---|---|
-20°C, 0.01 M Tris-HCl, pH 7.6, 0.5 M KCl | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | pyrophosphatase activity | Daucus carota | AMP + phosphate | - |
? | |
ATP + H2O | pyrophosphatase activity | Daucus carota | 5'-AMP + diphosphate | - |
? | |
DNA + H2O | enzyme hydrolyzes single-stranded DNA, no activity with double-stranded DNA | Daucus carota | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
additional information | - |
Daucus carota | ? | - |
? | |
NAD+ + H2O | pyrophosphatase activity | Daucus carota | 5'-AMP + NMN | - |
? | |
oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Daucus carota | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
p-nitrophenylthymidine 5'-phosphate + H2O | - |
Daucus carota | 5'-TMP + p-nitrophenol | - |
? | |
poly d(A-T)2 + H2O | - |
Daucus carota | ? | - |
? | |
polynucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Daucus carota | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
RNA + H2O | - |
Daucus carota | RNA(n-1) + nucleoside 5'-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable at, without loss of activity | Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Daucus carota |